Boosting tandem affinity purification of plant protein complexes

被引：94
|
作者：
Van Leene, Jelle [1 ,2 ]
Witters, Erwin [3 ,4 ]
Inze, Dirk [1 ,2 ]
De Jaeger, Geert [1 ,2 ]
机构：
[1] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium
[2] Univ Ghent, Dept Mol Genet, B-9052 Ghent, Belgium
[3] Univ Antwerp, Ctr Proteome Anal & Mass Spectrometry, B-2020 Antwerp, Belgium
[4] Vlaamse Instelling Technol Onderzoek, Flemish Inst Technol Res, B-2400 Mol, Belgium
来源：
TRENDS IN PLANT SCIENCE | 2008年 / 13卷 / 10期
关键词：
D O I：
10.1016/j.tplants.2008.08.002
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Protein-interaction mapping based on the tandem affinity purification (TAP) approach has been successfully established for several systems, such as yeast and mammalian cells. However, relatively few protein complex purifications have been reported for plants. Here, we highlight solutions for the pitfalls and propose a major breakthrough in the quest for a better TAP tag in plants.
引用
收藏
页码：517 / 520
页数：4
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