Spectroscopic diffraction phase microscopy

被引：60

作者：

Pham, Hoa ^{[1
]}

Bhaduri, Basanta ^{[1
]}

Ding, Huafeng ^{[1
]}

Popescu, Gabriel ^{[1
]}

机构：

[1] Univ Illinois, Dept Elect & Comp Engn, Beckman Inst Adv Sci & Technol, Quantitat Light Imaging Lab, Urbana, IL 61801 USA

来源：

OPTICS LETTERS | 2012年 / 37卷 / 16期

基金：

美国国家科学基金会;

关键词：

DIGITAL HOLOGRAPHIC MICROSCOPY; REFRACTIVE-INDEX; QUANTITATIVE PHASE; CELL MORPHOMETRY; HEMOGLOBIN; DISPERSION;

D O I：

10.1364/OL.37.003438

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present spectroscopic diffraction phase microscopy (sDPM) as a method capable of measuring quantitative phase images at multiple wavelengths. sDPM uses a spatial light modulator at the Fourier plane of a lens to select desired wavelengths from the white light illumination of a grating. The quantitative phase information at different wavelengths allows us to decouple the refractive index and the thickness from the phase shift induced by biological cells. We demonstrate the capability of the setup by dispersion measurements of microsphere beads and RBCs. (C) 2012 Optical Society of America

引用

收藏

页码：3438 / 3440

页数：3

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