Injury Activates Transient Olfactory Stem Cell States with Diverse Lineage Capacities

被引：75

作者：

Gadye, Levi ^{[1
]}

Das, Diya ^{[2
,3
]}

Sanchez, Michael A. ^{[2
,11
]}

Street, Kelly ^{[4
,9
]}

Baudhuin, Ariane ^{[2
]}

Wagner, Allon ^{[5
,9
]}

Cole, Michael B. ^{[6
,9
]}

Choi, Yoon Gi ^{[7
]}

Yosef, Nir ^{[5
,9
]}

Purdom, Elizabeth ^{[8
,9
]}

Dudoit, Sandrine ^{[3
,4
,8
,9
]}

Risso, Davide ^{[4
,10
]}

Ngai, John ^{[1
,2
,7
]}

Fletcher, Russell B. ^{[2
]}

机构：

[1] Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Berkeley Inst Data Sci, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Div Biostat, Berkeley, CA 94720 USA

[5] Univ Calif Berkeley, Dept Elect Engn & Comp Sci, Berkeley, CA 94720 USA

[6] Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA

[7] Univ Calif Berkeley, Funct Genom Lab QB3, Berkeley, CA 94720 USA

[8] Univ Calif Berkeley, Dept Stat, Berkeley, CA 94720 USA

[9] Univ Calif Berkeley, Ctr Computat Biol, Berkeley, CA 94720 USA

[10] Weill Cornell Med Coll, Dept Healthcare Policy & Res, New York, NY 10065 USA

[11] Vet Affairs Northern Calif Hlth Care Syst, Off Associate Director, Martinez, CA 94553 USA

来源：

CELL STEM CELL | 2017年 / 21卷 / 06期

关键词：

SELF-RENEWAL; DIFFERENTIAL EXPRESSION; BASAL-CELLS; HOMEOSTASIS; REVEALS; QUANTIFICATION; NORMALIZATION; PROGENITORS; MULTIPOTENT; EPITHELIUM;

D O I：

10.1016/j.stem.2017.10.014

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Tissue homeostasis and regeneration are mediated by programs of adult stem cell renewal and differentiation. However, the mechanisms that regulate stem cell fates under such widely varying conditions are not fully understood. Using single-cell techniques, we assessed the transcriptional changes associated with stem cell self-renewal and differentiation and followed the maturation of stem cell-derived clones using sparse lineage tracing in the regenerating mouse olfactory epithelium. Following injury, quiescent olfactory stem cells rapidly shift to activated, transient states unique to regeneration and tailored to meet the demands of injury-induced repair, including barrier formation and proliferation. Multiple cell fates, including renewed stem cells and committed differentiating progenitors, are specified during this early window of activation. We further show that Sox2 is essential for cells to transition from the activated to neuronal progenitor states. Our study highlights strategies for stem cell-mediated regeneration that may be conserved in other adult stem cell niches.

引用

页码：775 / +

页数：25

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