A Fluorescence-Based Genetic Screen to Study Retinal Degeneration in Drosophila

被引：11
|
作者：
Huang, Yu [1 ]
Xie, Jun [1 ,2 ]
Wang, Tao [1 ]
机构：
[1] Natl Inst Biol Sci, Beijing, Peoples R China
[2] China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China
来源：
PLOS ONE | 2015年 / 10卷 / 12期
关键词：
NERVOUS-SYSTEM; HUMAN SCO1; PROTEIN; TRANSPORT; RAB6; EYE; MUTANTS; ENCODES; CHANNEL; MODEL;
D O I：
10.1371/journal.pone.0144925
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The Drosophila visual system has been proved to be a powerful genetic model to study eye disease such as retinal degeneration. Here, we describe a genetic method termed "Rh1::GFP ey-flp/hid" that is based on the fluorescence of GFP-tagged major rhodopsin Rh1 in the eyes of living flies and can be used to monitor the integrity of photoreceptor cells. Through combination of this method and ERG recording, we examined a collection of 667 mutants and identified 18 genes that are required for photoreceptor cell maintenance, photoresponse, and rhodopsin synthesis. Our findings demonstrate that this "Rh1::GFP eyflp/hid" method enables high-throughput F1 genetic screens to rapidly and precisely identify mutations of retinal degeneration.
引用
收藏
页数：19
相关论文
共 50 条
  • [21] Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria
    Laudouze, Janis
    Point, Vanessa
    Achache, Wafaa
    Crauste, Celine
    Canaan, Stephane
    Santucci, Pierre
    FEBS LETTERS, 2025, 599 (04) : 488 - 501
  • [22] Fluorescence-based detection of field targets using an autonomous unmanned aerial vehicle system
    Kaye, Thomas G.
    Pittman, Michael
    METHODS IN ECOLOGY AND EVOLUTION, 2020, 11 (08): : 890 - 898
  • [23] Mutations in the splicing regulator Prp31 lead to retinal degeneration in Drosophila
    Hebbar, Sarita
    Lehmann, Malte
    Behrens, Sarah
    Haelsig, Catrin
    Leng, Weihua
    Yuan, Michaela
    Winkler, Sylke
    Knust, Elisabeth
    BIOLOGY OPEN, 2021, 10 (01):
  • [24] Drosophila melanogaster White Mutant w1118 Undergo Retinal Degeneration
    Jose Ferreiro, Maria
    Perez, Coralia
    Marchesano, Mariana
    Ruiz, Santiago
    Caputi, Angel
    Aguilera, Pedro
    Barrio, Rosa
    Cantera, Rafael
    FRONTIERS IN NEUROSCIENCE, 2018, 11
  • [25] A Fluorescence-based Method for Estimation of Oxygen Barrier Properties of Microspheres
    Kak, Atisheel
    Bajaj, Poonam R.
    Bhunia, Kanishka
    Nitin, Nitin
    Sablani, Shyam S.
    JOURNAL OF FOOD SCIENCE, 2019, 84 (03) : 532 - 539
  • [26] Fluorescence-Based Classification of Caribbean Coral Reef Organisms and Substrates
    Zawada, David G.
    Mazel, Charles H.
    PLOS ONE, 2014, 9 (01):
  • [27] Evaluation of Sensitivity of Fluorescence-Based Asbestos Detection by Correlative Microscopy
    Ishida, Takenori
    Alexandrov, Maxym
    Nishimura, Tomoki
    Minakawa, Kenji
    Hirota, Ryuichi
    Sekiguchi, Kiyoshi
    Kohyama, Norihiko
    Kuroda, Akio
    JOURNAL OF FLUORESCENCE, 2012, 22 (01) : 357 - 363
  • [28] Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli
    Standley, Melissa
    Allen, Jennifer
    Cervantes, Layla
    Lilly, Joshua
    Camps, Manel
    DNA REPAIR ENZYMES: CELL, MOLECULAR, AND CHEMICAL BIOLOGY, 2017, 591 : 159 - 186
  • [29] Small Molecule-Based Inducible Gene Therapies for Retinal Degeneration
    Datta, Shyamtanu
    Peng, Hui
    Hulleman, John D.
    RETINAL DEGENERATIVE DISEASES: MECHANISMS AND EXPERIMENTAL THERAPY, 2019, 1185 : 65 - 69
  • [30] Genetic Screen for Regulators of Lymph Gland Homeostasis and Hemocyte Maturation in Drosophila
    Tan, Kai Li
    Goh, Siow Chong
    Minakhina, Svetlana
    G3-GENES GENOMES GENETICS, 2012, 2 (03): : 393 - 405
12345