A Fluorescence-Based Genetic Screen to Study Retinal Degeneration in Drosophila

被引：11
|
作者：
Huang, Yu [1 ]
Xie, Jun [1 ,2 ]
Wang, Tao [1 ]
机构：
[1] Natl Inst Biol Sci, Beijing, Peoples R China
[2] China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China
来源：
PLOS ONE | 2015年 / 10卷 / 12期
关键词：
NERVOUS-SYSTEM; HUMAN SCO1; PROTEIN; TRANSPORT; RAB6; EYE; MUTANTS; ENCODES; CHANNEL; MODEL;
D O I：
10.1371/journal.pone.0144925
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The Drosophila visual system has been proved to be a powerful genetic model to study eye disease such as retinal degeneration. Here, we describe a genetic method termed "Rh1::GFP ey-flp/hid" that is based on the fluorescence of GFP-tagged major rhodopsin Rh1 in the eyes of living flies and can be used to monitor the integrity of photoreceptor cells. Through combination of this method and ERG recording, we examined a collection of 667 mutants and identified 18 genes that are required for photoreceptor cell maintenance, photoresponse, and rhodopsin synthesis. Our findings demonstrate that this "Rh1::GFP eyflp/hid" method enables high-throughput F1 genetic screens to rapidly and precisely identify mutations of retinal degeneration.
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收藏
页数：19
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