Regulation of B-Z Conformational Transition and Complex Formation with a Z-Form Binding Protein by Introduction of Constraint to Double-Stranded DNA by using a DNA Nanoscaffold

被引：9

作者：

Endo, Masayuki ^{[1
,3
]}

Inoue, Masahiro ^{[2
]}

Suzuki, Yuki ^{[2
,3
]}

Masui, Chigusa ^{[2
]}

Morinaga, Hironobu ^{[2
]}

Hidaka, Kumi ^{[1
]}

Sugiyama, Hiroshi ^{[1
,2
,3
]}

机构：

[1] Kyoto Univ, Inst Integrated Cell Mat Sci WPI iCeMS, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Grad Sch Sci, Dept Chem, Sakyo Ku, Kyoto 6068502, Japan

[3] CREST Japan Sci & Technol Corp JST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2013年 / 19卷 / 50期

关键词：

atomic force microscopy; DNA; proteins; single-molecule studies; VISUALIZATION; METHYLATION;

D O I：

10.1002/chem.201303830

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

B-Z transition: A constrained and rotatable double-stranded DNA, in which the rotational freedom was controlled by its placement into a DNA nanoscaffold, was designed (see scheme). The Z α β protein bound preferentially to the rotatable strand rather than the constrained strand, even using the same CG sequence. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：16887 / 16890

页数：4

共 18 条

[1] Intrinsic Z-DNA Is Stabilized by the Conformational Selection Mechanism of Z-DNA-Binding Proteins [J].

Bae, Sangsu ;

Kim, Doyoun ;

Kim, Kyeong Kyu ;

Kim, Yang-Gyun ;

Hohng, Sungchul .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (04) :668-671

[2] EFFECTS OF METHYLATION ON A SYNTHETIC POLYNUCLEOTIDE - THE B-Z TRANSITION IN POLY(DG-M5DC).POLY(DG-M5DC) [J].

BEHE, M ;

FELSENFELD, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (03) :1619-1623

[3]

Endo, 2010, ANGEW CHEM, V122, P9602

[4]

Endo M., 2012, ANGEW CHEM, V124, P10670

[5] Single-Molecule Visualization of the Hybridization and Dissociation of Photoresponsive Oligonucleotides and Their Reversible Switching Behavior in a DNA Nanostructure [J].

Endo, Masayuki ;

Yang, Yangyang ;

Suzuki, Yuki ;

Hidaka, Kumi ;

Sugiyama, Hiroshi .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (42) :10518-10522

[6] A Versatile DNA Nanochip for Direct Analysis of DNA Base-Excision Repair [J].

Endo, Masayuki ;

Katsuda, Yousuke ;

Hidaka, Kumi ;

Sugiyama, Hiroshi .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (49) :9412-9416

[7] Regulation of DNA Methylation Using Different Tensions of Double Strands Constructed in a Defined DNA Nanostructure [J].

Endo, Masayuki ;

Katsuda, Yousuke ;

Hidaka, Kumi ;

Sugiyama, Hiroshi .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (05) :1592-1597

[8] Modular bent DNAs: A new class of artificial DNAs with a protein binding ability [J].

Murata, Shunpei ;

Mizumura, Youko ;

Hino, Kaori ;

Ueno, Yoshihito ;

Ichikawa, Satoshi ;

Matsuda, Akira .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (34) :10300-+

[9] Z-DNA-binding proteins can act as potent effectors of gene expression in vivo [J].

Oh, DB ;

Kim, YG ;

Rich, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) :16666-16671

[10]

Rajendran A., 2012, ANGEW CHEM, V124, P898, DOI DOI 10.1002/ANGE.201102113

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