A Sensitive Aptasensor Based on a Hemin/G-Quadruplex-Assisted Signal Amplification Strategy for Electrochemical Detection of Gastric Cancer Exosomes

被引：321

作者：

Huang, Rongrong ^{[1
]}

He, Lei ^{[1
]}

Xia, Yanyan ^{[2
]}

Xu, Hongpan ^{[2
]}

Liu, Chang ^{[2
]}

Xie, Hui ^{[2
]}

Wang, Su ^{[2
]}

Peng, Lijun ^{[2
]}

Liu, Yufeng ^{[2
]}

Liu, Yuan ^{[1
]}

He, Nongyue ^{[1
,3
]}

Li, Zhiyang ^{[2
]}

机构：

[1] Southeast Univ, Natl Demonstrat Ctr Expt Biomed Engn Educ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

[2] Nanjing Univ, Med Sch, Affiliated Drum Tower Hosp, Dept Clin Lab, Nanjing 210008, Jiangsu, Peoples R China

[3] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Econ Forest Cultivat & Utilizat 2011 Collaborat I, Zhuzhou 412007, Peoples R China

来源：

SMALL | 2019年 / 15卷 / 19期

关键词：

aptamers; electrochemical; gastric cancer exosomes; rolling circle amplification; ROLLING CIRCLE AMPLIFICATION; VESICLES; APTAMERS;

D O I：

10.1002/smll.201900735

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Emerging evidence indicates that exosomes derived from gastric cancer cells enhance tumor migration and invasion through the modulation of the tumor microenvironment. However, it remains a major problem to detect cancer-specific exosomes due to technical and biological challenges. Most of the methods reported could not achieve efficient detection of tumor-derived exosomes in the background of normal exosomes. Herein, a label-free electrochemical aptasensor is presented for specific detection of gastric cancer exosomes. This platform contains an anti-CD63 antibody modified gold electrode and a gastric cancer exosome specific aptamer. The aptamer is linked to a primer sequence that is complementary to a G-quadruplex circular template. The presence of target exosomes could trigger rolling circle amplification and produce multiple G-quadruplex units. This horseradish peroxidase mimicking DNAzyme could catalyze the reduction of H2O2 and generate electrochemical signals. This aptasensor exhibits high selectivity and sensitivity toward gastric cancer exosomes with a detection limit of 9.54 x 10(2) mL(-1) and a linear response range from 4.8 x 10(3) to 4.8 x 10(6) exosomes per milliliter. Therefore, this electrochemical aptasensor is expected to become a useful tool for the early diagnosis of gastric cancer.

引用

页数：7

共 33 条

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