Long noncoding RNA expression profile of endothelial progenitor cells from deep vein thrombosis patients identified by microarray analysis

The aim of this study was to identify differentially expressed lncRNAs in endothelial progenitor cells (EPCs) derived from the peripheral blood of deep vein thrombosis (DVT) patients compared with healthy controls. First, EPCs were obtained from DVT patients and healthy controls. Then, LncRNA gene expression profile analysis was performed by microarray. Bioinformatic analyses (gene ontology, pathway and network analysis) were applied. Finally, qRT-PCR analysis was performed to validate the results of microarray in EPCs derived from 21 DVT patients and nine healthy subjects. We found 275 lncRNA and 363 mRNAs that were abnormally expressed in EPCs derived from DVT patients (fold change >= 2.0, P<0.05 and FDR<0.05) with the genome-wide lncRNAs and mRNAs expression profile analysis. We also found that TCONS_00013536, ENST00000577218, ENST00000511042 and TCONS_ 00013917 were up-regulated, whereas ENST00000544704 was down-regulated. Expression of these five lncRNAs was significantly correlated to their nearby coding genes. Together, our results indicated that the lncRNA expression profile in EPCs from DVT patients was dramatically changed compared with healthy controls, and we identified a series of novel DVT-related lncRNAs which will provide important insights about the lncRNAs in DVT pathogenesis and may provide a new diagnosis way for DVT.