Effects of grafting poly(ethylene oxide) on the amplification efficiency of a poly(dimethylsiloxane)-based flow-through PCR device

被引：8

作者：

Kuan, Iching ^{[1
]}

Gu, Weiguo ^{[1
]}

Wu, Jiancheng ^{[1
]}

Wei, Chehung ^{[2
]}

Chen, Koshao ^{[3
]}

Yu, Chiyang ^{[1
]}

机构：

[1] Tatung Univ, Dept Bioengn, Taipei 10452, Taiwan

[2] Tatung Univ, Dept Mech Engn, Taipei 10452, Taiwan

[3] Tatung Univ, Dept Mat Engn, Taipei 10452, Taiwan

来源：

CHEMICAL ENGINEERING JOURNAL | 2008年 / 143卷 / 1-3期

关键词：

poly(dimethylsiloxane); poly(ethylene oxide); polymerase chain reaction;

D O I：

10.1016/j.cej.2008.06.023

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The effects of grafting poly(ethylene oxide) (PEO) onto the surface of a microchannel on the amplification efficiency of a microfabricated device for polymerase chain reaction (PCR) were studied. The PCR device was composed of a poly(dimethylsiloxane) microchannel and a glass-heating chip. The PEO chains were grafted using neat silane or Pluronic (R) F127, and the presence of PEO was confirmed by water contact angle analysis and X-ray photoelectron spectroscopy. The surface treatments with neat silane and 10% (w/v) Pluronic (R) F127 resulted in an increase in the PCR amplification of a 298-bp DNA product by 2.2-fold and 3.9-fold, respectively, while 1.7-fold and 2.3-fold increases, respectively, were observed for a 1.1-kb DNA product. Both treatments could effectively enhance PCR efficiency even when DNA template concentration was decreased from 20 to 2 pg/mu l. Our results indicated that these simple surface treatments Could therefore be used routinely to enhance the performance of similar devices. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：326 / 330

页数：5

共 21 条

[1] Simple surface treatments to modify protein adsorption and cell attachment properties within a poly(dimethylsiloxane) micro-bioreactor [J].