Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method

被引：16
作者：
Zhao, Binan [4 ]
Yang, Dan [5 ]
Zhang, Yongchun [1 ]
Xu, Yan [3 ]
Zhao, Xiao [1 ,2 ]
Liang, Jieling [5 ]
Fan, Xiaorui [3 ]
Du, Yanan [3 ]
Zhu, Ziping [5 ]
Shi, Bin [1 ]
Zhang, Qi [3 ]
Zhang, Xiaoxia [3 ]
Cai, Youming [1 ]
Zhao, Kai [1 ,2 ]
机构：
[1] Shanghai Acad Agr Sci, Shanghai, Peoples R China
[2] Shanghai Acad Agr Sci, Key Lab Agr Genet & Breeding, Shanghai, Peoples R China
[3] Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
[4] XuZhou Med Univ, Dept Clin Med, Xuzhou, Peoples R China
[5] Taizhou Univ, Sch Life Sci, Taizhou, Peoples R China
来源：
ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 02期
关键词：
LAMP; ornamental plant; Potyvirus; virus detection; CUCUMBER MOSAIC-VIRUS; SYMPTOMLESS-VIRUS; INFECTING VIRUSES; LILIUM;
D O I：
10.1007/s00705-017-3618-4
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.
引用
收藏
页码：545 / 548
页数：4
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