Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method

被引：16

作者：

Zhao, Binan ^{[4
]}

Yang, Dan ^{[5
]}

Zhang, Yongchun ^{[1
]}

Xu, Yan ^{[3
]}

Zhao, Xiao ^{[1
,2
]}

Liang, Jieling ^{[5
]}

Fan, Xiaorui ^{[3
]}

Du, Yanan ^{[3
]}

Zhu, Ziping ^{[5
]}

Shi, Bin ^{[1
]}

Zhang, Qi ^{[3
]}

Zhang, Xiaoxia ^{[3
]}

Cai, Youming ^{[1
]}

Zhao, Kai ^{[1
,2
]}

机构：

[1] Shanghai Acad Agr Sci, Shanghai, Peoples R China

[2] Shanghai Acad Agr Sci, Key Lab Agr Genet & Breeding, Shanghai, Peoples R China

[3] Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China

[4] XuZhou Med Univ, Dept Clin Med, Xuzhou, Peoples R China

[5] Taizhou Univ, Sch Life Sci, Taizhou, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 02期

关键词：

LAMP; ornamental plant; Potyvirus; virus detection; CUCUMBER MOSAIC-VIRUS; SYMPTOMLESS-VIRUS; INFECTING VIRUSES; LILIUM;

D O I：

10.1007/s00705-017-3618-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.

引用

页码：545 / 548

页数：4

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