Inactivation of Shiga toxin-producing Escherichia coli O104:H4 using cold atmospheric pressure plasma

被引:23
作者
Baier, Matthias [1 ]
Janssen, Traute [2 ]
Wieler, Lothar H. [2 ]
Ehlbeck, Joerg [3 ]
Knorr, Dietrich [4 ]
Schlueter, Oliver [1 ]
机构
[1] Leibniz Inst Agr Engn, D-14469 Potsdam, Germany
[2] Free Univ Berlin, Inst Microbiol & Epizoot, Ctr Infect Med, D-14163 Berlin, Germany
[3] Leibniz Inst Plasma Sci & Technol, D-17489 Greifswald, Germany
[4] Berlin Univ Technol, Dept Food Biotechnol & Food Proc Engn, D-14195 Berlin, Germany
关键词
Plasma-jet; Escherichia coli O157:H7; Antibacterial treatment; Food safety; Fresh produce; Corn salad; HEMOLYTIC-UREMIC SYNDROME; 2011 GERMAN OUTBREAK; STRAIN;
D O I
10.1016/j.jbiosc.2015.01.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
From cultivation to the end of the post-harvest chain, heat-sensitive fresh produce is exposed to a variety of sources of pathogenic microorganisms. If contaminated, effective gentle means of sanitation are necessary to reduce bacterial pathogen load below their infective dose. The occurrence of rare or new serotypes raises the question of their tenacity to inactivation processes. In this study the antibacterial efficiency of cold plasma by an atmospheric pressure plasma-jet was examined against the Shiga toxin-producing outbreak strain Escherichia con O104:H4. Argon was transformed into non-thermal plasma at a power input of 8 W and a gas flow of 5 L min(-1). Basic tests were performed on polysaccharide gel discs, including the more common E. coli O157:H7 and non-pathogenic E. coli DSM 1116. At 5 mm treatment distance and 10(5) cfu cm(-2) initial bacterial count, plasma reduced E. coli O104:H4 after 60 s by 4.6 +/- 0.6 log, E. coli O157:H7 after 45 s by 4.5 +/- 0.6 log, and E. con DSM 1116 after 30 s by 4.4 +/- 1.1 log. On the surface of corn salad leaves, gentle plasma application at 17 mm reduced 10(4) cfu cm(-2) of E. con O104:H4 by 3.3 +/- 1.1 log after 2 min, whereas E. coli O157:H7 was inactivated by 3.2 +/- 1.1 log after 60 s. In conclusion, plasma treatment has the potential to reduce pathogens such as E. coli O104:H4 on the surface of fresh produce. However, a serotype-specific adaptation of the process parameters is required. (c) 2015, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码:275 / 279
页数:5
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