An improved deblocking agent for direct Fmoc solid-phase synthesis of peptide thioesters

被引：31
|
作者：
Bu, XZ
Xie, GY
Law, CW
Guo, ZH [1 ]
机构：
[1] Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China
[2] Hong Kong Univ Sci & Technol, Biotechnol Res Ctr, Kowloon, Hong Kong, Peoples R China
来源：
TETRAHEDRON LETTERS | 2002年 / 43卷 / 13期
关键词：
D O I：
10.1016/S0040-4039(02)00237-X
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
To synthesize peptide thioesters directly on a solid support for use as substrate analogues for thioesterases in non-ribosomal peptide synthases, we modified a reagent compatible with Fmoc solid-phase peptide synthesis that efficiently removes the protecting group while keeping the thioester intact. The deprotecting reagent, consisting of DBU and HOBt, was successfully used to prepare a decapeptide in high yield. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：2419 / 2422
页数：4
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