Long non-coding RNA FGF13-AS1 inhibits glycolysis and sternness properties of breast cancer cells through FGF13-AS1/IGF2BPs/Myc feedback loop

被引：127

作者：

Ma, Fei ^{[1
]}

Liu, Xu ^{[1
]}

Zhou, Shibo ^{[3
]}

Li, Wenjie ^{[1
]}

Liu, Chunxiao ^{[1
]}

Chadwick, Michelle ^{[4
]}

Qian, Cheng ^{[1
,2
]}

机构：

[1] Harbin Med Univ, Canc Hosp, Dept Breast Swgery, Harbin, Heilongjiang, Peoples R China

[2] Heilongjiang Acad Med Sci, Translat Med Res & Cooperat Ctr Northern China, Harbin, Heilongjiang, Peoples R China

[3] Harbin Med Univ, Affiliated Hosp 4, Dept Pharm, Harbin, Heilongjiang, Peoples R China

[4] Rutgers State Univ, Canc Inst New Jersey, New Brunswick, NJ USA

来源：

CANCER LETTERS | 2019年 / 450卷

基金：

中国国家自然科学基金;

关键词：

Lnc FGF13-AS1; Metabolism; Stemness; c-Myc; C-MYC ONCOGENE; PROLIFERATION; INTERPLAY; HYPOXIA; GENOME;

D O I：

10.1016/j.canlet.2019.02.008

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

LncRNAs have been proven to play crucial roles in various processes of breast cancer. LncRNA FGF13-AS1 has been identified as one of the 25 downregulated lncRNAs in breast cancer through analyzing data from two cohorts and TCGA by another group of our lab. In this study, we report that FGF13-AS1 expression is decreased in breast cancer tissue compared with corresponding normal tissue, and the downregulation of FGF13-AS1 is associated with poor prognosis. Functional studies show that FGF13-AS1 inhibits breast cancer cells proliferation, migration, and invasion by impairing glycolysis and sternness properties. Mechanistically, FGF13-AS1 reduces the half-life of c-Myc (Myc) mRNA by binding RNA-binding proteins, insulin-like growth factor 2 mRNA binding proteins (IGF2BPs) and disrupting the interaction between IGF2BPs and Myc mRNA. Furthermore, Myc transcriptionally inhibits FGF13-AS1, forming a feedback loop in this signaling pathway. These results reveal for the first time that FGF13-AS1 functions as a tumor suppressor by inhibiting glycolysis and sternness properties of breast cancer cells, and the FGF13-AS1/IGF2BPs/Myc feedback loop could be a novel therapeutic target for breast cancer patients.

引用

页码：63 / 75

页数：13

共 38 条

[1]

[Anonymous], 2018, ANTI-CANCER DRUG, DOI [DOI 10.3322/caac.20115, DOI 10.1097/CAD.0000000000000617]

[2] The c-Myc oncogene directly induces the H19 noncoding RNA by allele-specific binding to potentiate tumorigenesis [J].

Barsyte-Lovejoy, Dalia ;

Lau, Suzanne K. ;

Boutros, Paul C. ;

Khosravi, Fereshteh ;

Jurisica, Igor ;

Andrulis, Irene L. ;

Tsao, Ming S. ;

Penn, Linda Z. .

CANCER RESEARCH, 2006, 66 (10) :5330-5337

[3] Cytotopic localization by long noncoding RNAs [J].

Batista, Pedro J. ;

Chang, Howard Y. .

CURRENT OPINION IN CELL BIOLOGY, 2013, 25 (02) :195-199

[4] Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project [J].

Birney, Ewan ;

Stamatoyannopoulos, John A. ;

Dutta, Anindya ;

Guigo, Roderic ;

Gingeras, Thomas R. ;

Margulies, Elliott H. ;

Weng, Zhiping ;

Snyder, Michael ;

Dermitzakis, Emmanouil T. ;

Stamatoyannopoulos, John A. ;

Thurman, Robert E. ;

Kuehn, Michael S. ;

Taylor, Christopher M. ;

Neph, Shane ;

Koch, Christoph M. ;

Asthana, Saurabh ;

Malhotra, Ankit ;

Adzhubei, Ivan ;

Greenbaum, Jason A. ;

Andrews, Robert M. ;

Flicek, Paul ;

Boyle, Patrick J. ;

Cao, Hua ;

Carter, Nigel P. ;

Clelland, Gayle K. ;

Davis, Sean ;

Day, Nathan ;

Dhami, Pawandeep ;

Dillon, Shane C. ;

Dorschner, Michael O. ;

Fiegler, Heike ;

Giresi, Paul G. ;

Goldy, Jeff ;

Hawrylycz, Michael ;

Haydock, Andrew ;

Humbert, Richard ;

James, Keith D. ;

Johnson, Brett E. ;

Johnson, Ericka M. ;

Frum, Tristan T. ;

Rosenzweig, Elizabeth R. ;

Karnani, Neerja ;

Lee, Kirsten ;

Lefebvre, Gregory C. ;

Navas, Patrick A. ;

Neri, Fidencio ;

Parker, Stephen C. J. ;

Sabo, Peter J. ;

Sandstrom, Richard ;

Shafer, Anthony .

NATURE, 2007, 447 (7146) :799-816

[5] Correlation of amplification and overexpression of the c-myc oncogene in high-grade breast cancer:: FISH, in situ hybridisation and immunohistochemical analyses [J].

Blancato, J ;

Singh, B ;

Liu, A ;

Liao, DJ ;

Dickson, RB .

BRITISH JOURNAL OF CANCER, 2004, 90 (08) :1612-1619

[6] The PVT-1 oncogene is a myc protein target is overexpressed in transformed cells [J].

Carramusa, Letizia ;

Contino, Flavia ;

Ferro, Arianna ;

Minafra, Luigi ;

Perconti, Giovanni ;

Giallongo, Agata ;

Feo, Salvatore .

JOURNAL OF CELLULAR PHYSIOLOGY, 2007, 213 (02) :511-518

[7] Hypoxia and metabolism - Opinion - The interplay between MYC and HIF in cancer [J].

Dang, Chi V. ;

Kim, Jung-whan ;

Gao, Ping ;

Yustein, Jason .

NATURE REVIEWS CANCER, 2008, 8 (01) :51-56

[8] MYC, Metabolism, Cell Growth, and Tumorigenesis [J].

Dang, Chi V. .

COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, 2013, 3 (08)

[9] RETRACTED: Long Non-Coding RNA HOTAIR Regulates the Proliferation, Self-Renewal Capacity, Tumor Formation and Migration of the Cancer Stem-Like Cell (CSC) Subpopulation Enriched from Breast Cancer Cells (Retracted article. See vol. 19, 2024) [J].

Deng, Jia ;

Yang, Mengchang ;

Jiang, Rong ;

An, Ning ;

Wang, Xiaoshan ;

Liu, Bin .

PLOS ONE, 2017, 12 (01)

[10] Role of long non-coding RNAs in glucose metabolism in cancer [J].

Fan, Chunmei ;

Tang, Yanyan ;

Wang, Jinpeng ;

Xiong, Fang ;

Guo, Can ;

Wang, Yumin ;

Zhang, Shanshan ;

Gong, Zhaojian ;

Wei, Fang ;

Yang, Liting ;

He, Yi ;

Zhou, Ming ;

Li, Xiaoling ;

Li, Guiyuan ;

Xiong, Wei ;

Zeng, Zhaoyang .

MOLECULAR CANCER, 2017, 16

← 1 2 3 4 →