Antiphosphatidylserine/prothrombin antibodies (aPS/PT) as potential diagnostic markers and risk predictors of venous thrombosis and obstetric complications in antiphospholipid syndrome

被引：93

作者：

Shi, Hui ^{[2
]}

Zheng, Hui ^{[3
]}

Yin, Yu-Feng ^{[2
]}

Hu, Qiong-Yi ^{[2
]}

Teng, Jia-Lin ^{[2
]}

Sun, Yue ^{[2
]}

Liu, Hong-Lei ^{[2
]}

Cheng, Xiao-Bing ^{[2
]}

Ye, Jun-Na ^{[2
]}

Su, Yu-Tong ^{[2
]}

Wu, Xin-Yao ^{[2
]}

Zhou, Jin-Feng ^{[4
]}

Norman, Gary L. ^{[5
]}

Gong, Hui-Yun ^{[1
]}

Shi, Xin-Ming ^{[1
]}

Peng, Yi-Bing ^{[1
]}

Wang, Xue-Feng ^{[1
]}

Yang, Cheng-De ^{[2
]}

机构：

[1] Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Dept Lab Med, 197 Ruijin Second Rd, Shanghai 200025, Peoples R China

[2] Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Dept Rheumatol & Immunol, 197 Ruijin Second Rd, Shanghai 200025, Peoples R China

[3] Taishan Med Univ, Affiliated Hosp, Dept Rheumatol & Immunol, Tai An, Shandong, Peoples R China

[4] Werfen Med Device Trading Beijing Co Ltd, Beijing, Peoples R China

[5] INOVA Diagnost Inc, San Diego, CA USA

来源：

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 2018年 / 56卷 / 04期

基金：

中国国家自然科学基金;

关键词：

antiphosphatidylserine/prothrombin antibodies; antiphospholipid syndrome; lupus anticoagulant; seronegative APS; PHOSPHATIDYLSERINE-PROTHROMBIN COMPLEX; INTERNATIONAL CONSENSUS STATEMENT; CLASSIFICATION CRITERIA; REVISED CRITERIA; AUTOANTIBODIES; ASSOCIATION; UPDATE; TESTS;

D O I：

10.1515/cclm-2017-0502

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The aim of the study was to determine the prevalence and clinical associations of antiphosphatidylserine/prothrombin antibodies (aPS/PT) with thrombosis and pregnancy loss in Chinese patients with antiphospholipid syndrome (APS) and seronegative APS (SNAPS). Methods: One hundred and eighty six Chinese patients with APS (67 primary, 119 secondary), 48 with SNAPS, 176 disease controls (79 systemic lupus erythematosus [SLE], 29 Sjogren's syndrome [SS], 30 ankylosing spondylitis [AS], 38 rheumatoid arthritis [RA]) and 90 healthy donors were examined. IgG and IgM aPS/PT, IgG/IgM/IgA -anticardiolipin (aCL) and IgG/IgM/IgA anti-beta(2)-glycoprotein I (anti-beta(2)GPI) antibodies were tested by ELISA. Results: One hundred and sixty (86.0%) of APS patients were positive for at least one aPS/PT isotype. One hundred and thirty five (72.6%) were positive for IgG aPS/PT, 124/186 (66.7%) positive for IgM aPS/PT and 99 (53.2%) positive for both. Approximately half of the SNAPS patients were positive for IgG and/or IgM aPS/PT. Highly significant associations between IgG aPS/PT and venous thrombotic events (odds ratio [OR] = 6.72) and IgG/IgM aPS/PT and pregnancy loss (OR = 9.44) were found. Levels of IgM aPS/PT were significantly different in APS patients with thrombotic manifestations and those with fetal loss (p = 0.014). The association between IgG/IgM aPS/PT and lupus anticoagulant (LAC) was highly significant (p < 0.001). When both were positive, the OR for APS was 101.6. Notably, 91.95% (80/87) of LAC-positive specimens were positive for IgG and/or IgM aPS/PT, suggesting aPS/PT is an effective option when LAC testing is not available. Conclusions: Anti-PS/PT antibody assays demonstrated high diagnostic performance for Chinese patients with APS, detected some APS patients negative for criteria markers and may serve as potential risk predictors for venous thrombosis and obstetric complications.

引用

页码：614 / 624

页数：11

共 37 条

[11]

BRANDT JT, 1995, THROMB HAEMOSTASIS, V74, P1185

[12] ANTICARDIOLIPIN ANTIBODIES (ACA) DIRECTED NOT TO CARDIOLIPIN BUT TO A PLASMA-PROTEIN COFACTOR [J].

GALLI, M ;

COMFURIUS, P ;

MAASSEN, C ;

HEMKER, HC ;

DEBAETS, MH ;

VANBREDAVRIESMAN, PJC ;

BARBUI, T ;

ZWAAL, RFA ;

BEVERS, EM .

LANCET, 1990, 335 (8705) :1544-1547

[13] Antiprothrombin antibodies: Detection and clinical significance in the antiphospholipid syndrome [J].

Galli, M ;

Barbui, T .

BLOOD, 1999, 93 (07) :2149-2157

[14] Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus [J].

Hochberg, MC .

ARTHRITIS AND RHEUMATISM, 1997, 40 (09) :1725-1725

[15] Relationship between antiphosphatidylserine/prothrombin and conventional antiphospholipid antibodies in primary antiphospholipid syndrome [J].

Hoxha, Ariela ;

Ruffatti, Amelia ;

Mattia, Elena ;

Meneghel, Lauro ;

Tonello, Marta ;

Salvan, Elisa ;

Pengo, Vittorio ;

Punzi, Leonardo .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2015, 53 (08) :1265-1270

[16] Seronegative antiphospholipid syndrome [J].

Hughes, GRV ;

Khamashta, MA .

ANNALS OF THE RHEUMATIC DISEASES, 2003, 62 (12) :1127-1127

[17] Autoantibodies against phosphatidylserine, prothrombin and phosphatidylserine-prothrombin complex: Identical or distinct diagnostic tools for antiphospholipid syndrome? [J].

Jaskowski, Troy D. ;

Wilson, Andrew R. ;

Hill, Harry R. ;

Branch, Ware D. ;

Tebo, Anne E. .

CLINICA CHIMICA ACTA, 2009, 410 (1-2) :19-24

[18] High titer of anti-phosphatidylserine-prothrombin complex antibodies in patients with cutaneous polyarteritis nodosa [J].

Kawakami, Tamihiro ;

Yamazaki, Masahide ;

Mizoguchi, Masako ;

Soma, Yoshinao .

ARTHRITIS & RHEUMATISM-ARTHRITIS CARE & RESEARCH, 2007, 57 (08) :1507-1513

[19] Treatment of catastrophic antiphospholipid syndrome [J].

Kazzaz, Nayef M. ;

McCune, W. Joseph ;

Knight, Jason S. .

CURRENT OPINION IN RHEUMATOLOGY, 2016, 28 (03) :218-227

[20] International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS) [J].

Miyakis, S ;

Lockshin, MD ;

Atsumi, T ;

Branch, DW ;

Brey, RL ;

Cervera, R ;

Derksen, RHWM ;

De Groot, PG ;

Koike, T ;

Meroni, PL ;

Reber, G ;

Shoenfeld, Y ;

Tincani, A ;

Vlachoyiannopoulos, PG ;

Krilis, SA .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2006, 4 (02) :295-306

← 1 2 3 4 →