Design of oligonucleotide arrays at interfaces

被引:89
作者
Boncheva, M
Scheibler, L
Lincoln, P
Vogel, H
Åkerman, B
机构
[1] Chalmers Univ Technol, Dept Phys Chem, S-41296 Gothenburg, Sweden
[2] Univ Lausanne, Inst Chim Organ, CH-1015 Lausanne, Switzerland
[3] Ecole Polytech Fed Lausanne, Lab Chim Phys Polymeres & Membranes, CH-1015 Lausanne, Switzerland
关键词
D O I
10.1021/la981702t
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The surface attachment and detection of DNA probes are essential in the design of nucleic acid-based biosensors. A new strategy for the covalent immobilization of single-stranded oligonucleotides on gold-covered planar supports is presented. Optimization of the surface density in the resulting DNA arrays permits a high hybridization efficiency to be achieved. Surface plasmon resonance and, for the first time, ATR-FTIR spectroscopy are used to follow in situ the oligonucleotide layer formation and the subsequent complementary strand hybridization. Such well-defined, covalently immobilized oligonucleotide arrays can find application in the development of novel DNA-based sensors for mutation detection and gene mapping as well as in studies of nucleic acid-ligand interactions.
引用
收藏
页码:4317 / 4320
页数:4
相关论文
共 22 条
[1]   Fiber-optic evanescent wave biosensor for the detection of oligonucleotides [J].
Abel, AP ;
Weller, MG ;
Duveneck, GL ;
Ehrat, M ;
Widmer, HM .
ANALYTICAL CHEMISTRY, 1996, 68 (17) :2905-2912
[2]   BATHOPHENANTHROLINE-RUTHENIUM(II) COMPLEXES AS NON-RADIOACTIVE LABELS FOR OLIGONUCLEOTIDES WHICH CAN BE MEASURED BY TIME-RESOLVED FLUORESCENCE TECHNIQUES [J].
BANNWARTH, W ;
SCHMIDT, D ;
STALLARD, RL ;
HORNUNG, C ;
KNORR, R ;
MULLER, F .
HELVETICA CHIMICA ACTA, 1988, 71 (08) :2085-2099
[3]  
BIER FF, 1997, FRONTIERS BIOSENSORI, V1, P97
[4]   Formation of stable polypeptide monolayers at interfaces: Controlling molecular conformation and orientation [J].
Boncheva, M ;
Vogel, H .
BIOPHYSICAL JOURNAL, 1997, 73 (02) :1056-1072
[5]  
BRANDRUP J, 1989, POLYM HDB, V2, P462
[6]   Quartz crystal microbalance study of DNA immobilization and hybridization for nucleic acid sensor development [J].
Caruso, F ;
Rodda, E ;
Furlong, DF ;
Niikura, K ;
Okahata, Y .
ANALYTICAL CHEMISTRY, 1997, 69 (11) :2043-2049
[7]   Adsorption and surface diffusion of DNA oligonucleotides at liquid/solid interfaces [J].
Chan, V ;
Graves, DJ ;
Fortina, P ;
McKenzie, SE .
LANGMUIR, 1997, 13 (02) :320-329
[8]   Attenuated total reflection Fourier transform infrared spectroscopic characterization of fluid lipid bilayers tethered to solid supports [J].
Cheng, YL ;
Boden, N ;
Bushby, RJ ;
Clarkson, S ;
Evans, SD ;
Knowles, PF ;
Marsh, A ;
Miles, RE .
LANGMUIR, 1998, 14 (04) :839-844
[9]   SELF-CONDENSATION OF ACETAMIDOACETALDEHYDE AND OF AMINOACETONE [J].
CORNFORTH, JW .
JOURNAL OF THE CHEMICAL SOCIETY, 1958, (MAR) :1174-1176
[10]   Characterization of DNA probes immobilized on gold surfaces [J].
Herne, TM ;
Tarlov, MJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (38) :8916-8920