Bio-Click Chemistry: Enzymatic Functionalization of PEGylated Capsules for Targeting Applications

被引：63
作者：
Leung, Melissa K. M. [2 ]
Hagemeyer, Christoph E. [1 ,4 ,5 ]
Johnston, Angus P. R. [2 ]
Gonzales, Catalina [2 ]
Kamphuis, Marloes M. J. [2 ]
Ardipradja, Katie [1 ]
Such, Georgina K. [2 ]
Peter, Karlheinz [1 ,3 ,5 ]
Caruso, Frank [2 ]
机构：
[1] Baker IDI Heart & Diabet Inst, Melbourne, Vic, Australia
[2] Univ Melbourne, Dept Chem & Biomol Engn, Melbourne, Vic 3010, Australia
[3] Monash Univ, Dept Med, Clayton, Vic 3800, Australia
[4] Monash Univ, Dept Clin Haematol, Clayton, Vic 3800, Australia
[5] Monash Univ, Dept Immunol, Clayton, Vic 3800, Australia
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 29期
基金：
澳大利亚研究理事会; 英国医学研究理事会;
关键词：
drug delivery; enzyme catalysis; functionalization; polymers; scFv; STAPHYLOCOCCUS-AUREUS SORTASE; MEDIATED LIGATION; SURFACE-PROTEINS; FUSION PROTEINS; CELL-WALL; PEPTIDE; NANOPARTICLES; CONJUGATION; STRATEGY; SRTA;
D O I：
10.1002/anie.201203612
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
All sorted: The enzyme Sortase A was used to catalyze functionalization of PEGylated capsules with an activation-specific anti-platelet single-chain antibody (scFv). This enzymatic method allows fast, covalent, and site-directed functionalization of delivery vehicles under mild conditions. Activation-specific anti-platelet scFv-coated PEGylated capsules exhibited a high level of selective binding to thrombi, thus suggesting their potential for thrombosis therapy. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：7132 / 7136
页数：5
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