Bio-Click Chemistry: Enzymatic Functionalization of PEGylated Capsules for Targeting Applications

被引：63

作者：

Leung, Melissa K. M. ^{[2
]}

Hagemeyer, Christoph E. ^{[1
,4
,5
]}

Johnston, Angus P. R. ^{[2
]}

Gonzales, Catalina ^{[2
]}

Kamphuis, Marloes M. J. ^{[2
]}

Ardipradja, Katie ^{[1
]}

Such, Georgina K. ^{[2
]}

Peter, Karlheinz ^{[1
,3
,5
]}

Caruso, Frank ^{[2
]}

机构：

[1] Baker IDI Heart & Diabet Inst, Melbourne, Vic, Australia

[2] Univ Melbourne, Dept Chem & Biomol Engn, Melbourne, Vic 3010, Australia

[3] Monash Univ, Dept Med, Clayton, Vic 3800, Australia

[4] Monash Univ, Dept Clin Haematol, Clayton, Vic 3800, Australia

[5] Monash Univ, Dept Immunol, Clayton, Vic 3800, Australia

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 29期

基金：

英国医学研究理事会; 澳大利亚研究理事会;

关键词：

drug delivery; enzyme catalysis; functionalization; polymers; scFv; STAPHYLOCOCCUS-AUREUS SORTASE; MEDIATED LIGATION; SURFACE-PROTEINS; FUSION PROTEINS; CELL-WALL; PEPTIDE; NANOPARTICLES; CONJUGATION; STRATEGY; SRTA;

D O I：

10.1002/anie.201203612

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

All sorted: The enzyme Sortase A was used to catalyze functionalization of PEGylated capsules with an activation-specific anti-platelet single-chain antibody (scFv). This enzymatic method allows fast, covalent, and site-directed functionalization of delivery vehicles under mild conditions. Activation-specific anti-platelet scFv-coated PEGylated capsules exhibited a high level of selective binding to thrombi, thus suggesting their potential for thrombosis therapy. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：7132 / 7136

页数：5

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