In situ detection of miRNAs in animal embryos using LNA-modified oligonucteotide probes

被引：474

作者：

Kloosterman, WP

Wienholds, E

de Bruijn, E

Kauppinen, S

Plasterk, RHA

机构：

[1] Netherlands Inst Dev Biol, Hubrecht Lab, Ctr Biomed Genet, NL-3584 CT Utrecht, Netherlands

[2] Univ Copenhagen, Inst Med Biochem & Genet, Wilhelm Johannsen Ctr Funct Genome Res, DK-2200 Copenhagen, Denmark

来源：

NATURE METHODS | 2006年 / 3卷 / 01期

关键词：

D O I：

10.1038/NMETH843

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using Locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.

引用

收藏

页码：27 / 29

页数：3

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