In situ detection of miRNAs in animal embryos using LNA-modified oligonucteotide probes

被引：474
作者：
Kloosterman, WP
Wienholds, E
de Bruijn, E
Kauppinen, S
Plasterk, RHA
机构：
[1] Netherlands Inst Dev Biol, Hubrecht Lab, Ctr Biomed Genet, NL-3584 CT Utrecht, Netherlands
[2] Univ Copenhagen, Inst Med Biochem & Genet, Wilhelm Johannsen Ctr Funct Genome Res, DK-2200 Copenhagen, Denmark
来源：
NATURE METHODS | 2006年 / 3卷 / 01期
关键词：
D O I：
10.1038/NMETH843
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using Locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
引用
收藏
页码：27 / 29
页数：3
相关论文
共 12 条
  • [1] Identification of hundreds of conserved and nonconserved human microRNAs
    Bentwich, I
    Avniel, A
    Karov, Y
    Aharonov, R
    Gilad, S
    Barad, O
    Barzilai, A
    Einat, P
    Einav, U
    Meiri, E
    Sharon, E
    Spector, Y
    Bentwich, Z
    [J]. NATURE GENETICS, 2005, 37 (07) : 766 - 770
  • [2] Phylogenetic shadowing and computational identification of human microRNA genes
    Berezikov, E
    Guryev, V
    van de Belt, J
    Wienholds, E
    Plasterk, RHA
    Cuppen, E
    [J]. CELL, 2005, 120 (01) : 21 - 24
  • [3] MicroRNAs regulate brain morphogenesis in zebrafish
    Giraldez, AJ
    Cinalli, RM
    Glasner, ME
    Enright, AJ
    Thomson, JM
    Baskerville, S
    Hammond, SM
    Bartel, DP
    Schier, AF
    [J]. SCIENCE, 2005, 308 (5723) : 833 - 838
  • [4] The microRNA Registry
    Griffiths-Jones, S
    [J]. NUCLEIC ACIDS RESEARCH, 2004, 32 : D109 - D111
  • [5] MicroRNA expression profiles classify human cancers
    Lu, J
    Getz, G
    Miska, EA
    Alvarez-Saavedra, E
    Lamb, J
    Peck, D
    Sweet-Cordero, A
    Ebet, BL
    Mak, RH
    Ferrando, AA
    Downing, JR
    Jacks, T
    Horvitz, HR
    Golub, TR
    [J]. NATURE, 2005, 435 (7043) : 834 - 838
  • [6] MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression
    Mansfield, JH
    Harfe, BD
    Nissen, R
    Obenauer, J
    Srineel, J
    Chaudhuri, A
    Farzan-Kashani, R
    Zuker, M
    Pasquinelli, AE
    Ruvkun, G
    Sharp, PA
    Tabin, CJ
    McManus, MT
    [J]. NATURE GENETICS, 2004, 36 (10) : 1079 - 1083
  • [7] Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation
    Sempere, LF
    Freemantle, S
    Pitha-Rowe, I
    Moss, E
    Dmitrovsky, E
    Ambros, V
    [J]. GENOME BIOLOGY, 2004, 5 (03)
  • [8] A custom microarray platform for analysis of microRNA gene expression
    Thomson, JM
    Parker, J
    Perou, CM
    Hammond, SM
    [J]. NATURE METHODS, 2004, 1 (01) : 47 - 53
  • [9] LNA (Locked nucleic acid): High-affinity targeting of complementary RNA and DNA
    Vester, B
    Wengel, J
    [J]. BIOCHEMISTRY, 2004, 43 (42) : 13233 - 13241
  • [10] MicroRNA function in animal development
    Wienholds, E
    Plasterk, RHA
    [J]. FEBS LETTERS, 2005, 579 (26) : 5911 - 5922
12