Selective Zinc(II)-Ion Fluorescence Sensing by a Functionalized Mesoporous Material Covalently Grafted with a Fluorescent Chromophore and Consequent Biological Applications

被引:201
作者
Sarkar, Krishanu [1 ,2 ]
Dhara, Koushik [3 ]
Nandi, Mahasweta [1 ,2 ]
Roy, Partha [3 ]
Bhaumik, Asim [1 ,2 ]
Banerjee, Pradyot [3 ]
机构
[1] Indian Assoc Cultivat Sci, Dept Mat Sci, Kolkata 700032, India
[2] Indian Assoc Cultivat Sci, Ctr Adv Mat, Kolkata 700032, India
[3] Indian Assoc Cultivat Sci, Dept Inorgan Chem, Kolkata 700032, India
关键词
RESPONSIVE CONTROLLED-RELEASE; PANCREATIC BETA-CELLS; TOXIC METAL-IONS; SILICA NANOPARTICLES; HIPPOCAMPAL SLICES; NEURONAL ZINC; 2-PHOTON MICROSCOPY; TUNABLE CHEMOSENSOR; INTRACELLULAR ZINC; SENSOR MOLECULES;
D O I
10.1002/adfm.200800888
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A highly ordered 2D-hexagonal mesoporous silica material is functionalized with 3-aminopropyltriethoxysilane. This organically modified mesoporous material is grafted with a dialdehyde fluorescent chromophore, 4-methyl-2,6-diformyl phenol. Powder X-ray diffraction, transmission electron microscopy, N-2 sorption, Fourier transform infrared spectroscopy, and UV-visible absorption and emission have been employed to characterize the material. This material shows excellent selective Zn2+ sensing, which is due to the fluorophore moiety present at its surface. Fluorescence measurements reveal that the emission intensity of the Zn2+-bound mesoporous material increases significantly upon addition of various concentrations of Zn2+, while the introduction of other biologically relevant (Ca2+, Mg2+, Na+, and K+) and environmentally hazardous transition-metal Ions results in either unchanged or weakened intensity. The enhancement of fluorescence is attributed to the strong covalent binding of Zn2+, evident from the large binding constant value (0.87 x 10(4) M-1). Thus, this functionalized mesoporous material grafted with the fluorescent chromophore could monitor or recognize Zn2+ from a mixture of ions that contains Zn2+ even in trace amounts and can be considered as a selective fluorescent probe. We have examined the application of this mesoporous zinc(II) sensor to cultured living cells (A375 human melanoma and human cervical cancer cell, HeLa) by fluorescence microscopy.
引用
收藏
页码:223 / 234
页数:12
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