Cell infection within a microfluidic device using virus gradients

被引：61
作者：
Walker, GM
Ozers, MS
Beebe, DJ [1 ]
机构：
[1] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA
[2] PanVera Invitrogen, Madison, WI 53719 USA
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2004年 / 98卷 / 2-3期
关键词：
microfluidics; baculovirus expression system; PDMS; microchannel; concentration gradient;
D O I：
10.1016/j.snb.2003.10.014
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A microfluidic device has been developed which allows cells to be infected at many different concentrations of virus within a microscale environment. Diffusion and laminar flow were used to create a concentration gradient of virus particles over cells attached to the bottom of a microchannel. The expression of green fluorescent protein (GFP) was monitored optically in situ over several days. In characterizing non-gradient infection at the microscale, average multiplicity of infections (MOIs) ranging from 8 to 25 were used and reduced GFP expression levels, compared to accepted macroscale values, were observed. Cells infected with virus gradients also displayed reduced expression levels but their location within the microchannel followed the same trend as the virus gradient. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：347 / 355
页数：9
相关论文
共 26 条
[1]  
Bird R.B., 2006, TRANSPORT PHENOMENA, Vsecond, DOI 10.1002/aic.690070245
[2]   Diffusion-based extraction in a microfabricated device [J].
Brody, JP ;
Yager, P .
SENSORS AND ACTUATORS A-PHYSICAL, 1997, 58 (01) :13-18
[3]   Clinical potential of microchip capillary electrophoresis systems [J].
Colyer, CL ;
Tang, T ;
Chiem, N ;
Harrison, DJ .
ELECTROPHORESIS, 1997, 18 (10) :1733-1741
[4]   Generation of gradients having complex shapes using microfluidic networks [J].
Dertinger, SKW ;
Chiu, DT ;
Jeon, NL ;
Whitesides, GM .
ANALYTICAL CHEMISTRY, 2001, 73 (06) :1240-1246
[5]   Gradients of substrate-bound laminin orient axonal specification of neurons [J].
Dertinger, SKW ;
Jiang, XY ;
Li, ZY ;
Murthy, VN ;
Whitesides, GM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12542-12547
[6]  
Dolník V, 2000, ELECTROPHORESIS, V21, P41, DOI 10.1002/(SICI)1522-2683(20000101)21:1<41::AID-ELPS41>3.0.CO
[7]  
2-7
[8]   Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) [J].
Duffy, DC ;
McDonald, JC ;
Schueller, OJA ;
Whitesides, GM .
ANALYTICAL CHEMISTRY, 1998, 70 (23) :4974-4984
[9]   A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion [J].
Hansen, CL ;
Skordalakes, E ;
Berger, JM ;
Quake, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) :16531-16536
[10]   MICROMACHINING A MINIATURIZED CAPILLARY ELECTROPHORESIS-BASED CHEMICAL-ANALYSIS SYSTEM ON A CHIP [J].
HARRISON, DJ ;
FLURI, K ;
SEILER, K ;
FAN, ZH ;
EFFENHAUSER, CS ;
MANZ, A .
SCIENCE, 1993, 261 (5123) :895-897
123