Direct visualisation of plasmid DNA in individual chromatography adsorbent particles by confocal scanning laser microscopy

被引：77

作者：

Ljunglöf, A ^{[1
]}

Bergvall, P ^{[1
]}

Bhikhabhai, R ^{[1
]}

Hjorth, R ^{[1
]}

机构：

[1] Amersham Pharm Biotech, SE-75184 Uppsala, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 844卷 / 1-2期

关键词：

confocal scanning laser microscopy; DNA;

D O I：

10.1016/S0021-9673(99)00386-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Confocal microscopy was used for the measurement of plasmid DNA adsorbed to individual adsorbent particles intended for anion-exchange and triple helix affinity chromatography. Plasmid DNA was visualized with the fluorescent dye YOYO-1, that forms a highly fluorescent complex with double stranded DNA. Confocal images were translated into fluorescence intensity profiles and the distribution of plasmid DNA in the particles was measured. The results revealed that adsorption of plasmid DNA mainly takes place in an outer layer of the particles. The described procedure can also be advantageously used to demonstrate triple helix formation between plasmid DNA and immobilized oligonucleotides. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：129 / 135

页数：7

共 17 条

[1]

*AM PHARM BIOT, 18112951 AM PHARM BI

[2] 3-DIMENSIONAL IMAGING OF DNA FRAGMENTS DURING ELECTROPHORESIS USING A CONFOCAL DETECTOR [J].