Comparative study between fructose 1-6 bisphosphate and histidine-tryptophan-ketoglutarate in liver preservation in rats submitted to total cold ischemia

被引：1

作者：

Smecellato, Fernanda Bombonato ^{[1
]}

Benfatti Marsilli, Lucas Ricardo ^{[1
]}

Nakamura, Julia Eico ^{[1
]}

Jordani, Maria Cecilia ^{[2
]}

Barbosa Evora, Paulo Roberto ^{[3
]}

Castro-e-Silva, Orlando ^{[4
]}

机构：

[1] Fac Med Marilia FAMEMA, Marilia, SP, Brazil

[2] Univ Sao Paulo FMRP USP, Dept Surg & Anat, Div Digest Surg, Fac Med Ribeirao Preto, Ribeirao Preto, SP, Brazil

[3] FMRP USP, Dept Surg & Anat, Div Thorac & Cardiovasc Surg, Ribeirao Preto, SP, Brazil

[4] FMRP USP, Dept Surg & Anat, Ribeirao Preto, SP, Brazil

来源：

ACTA CIRURGICA BRASILEIRA | 2020年 / 35卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

Cold lschemia; Organ Preservation; Liver Transplantation; Rats; STORAGE SOLUTION; FRUCTOSE-1,6-BISPHOSPHATE; FRUCTOSE-1,6-DIPHOSPHATE; OXYGEN;

D O I：

10.1590/s0102-865020200060000003

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Purpose: To compare Fructose-1,6-Bisphosphate (FBP) to Histidine-Tryptophan-Ketoglutarate (HTK) in liver preservation at cold ischemia. Methods: Male rats (Sprague-Dawley: 280-340g) divided into three groups (n=7): Control; Fructose-1,6-bisphosphate (FBP); Histidine-Tryptophan-Ketoglutarate (HTK). Animals underwent la parotomythoracotomy for perfusion of livers with saline. Livers were removed and deposited into solutions. Mitochondria were isolated to determine State 3 (S3), State 4 (S4), Respiratory Control Ratio (RCR) and Swelling (5). Liver enzymes (AST, ALT, LDH) were determined in solution. At tissue, Malondialdehyde (MDA) and Nitrate (NOx) were determined. All parameters were analyzed at 0.6 and 24 hours of hypothermic preservation. Statistics analysis were made by Mann-Whitney test (p<0.05). Results: Regarding ALT, there was a difference between FBP-6h/HTK-6h, lower in HTK. Regarding AST, there was a significant difference between FBP-24h/HTK-24h, lower in FBP. Regarding NOx, there was a difference between Oh and 6h, as well as Oh and 24h for both solutions. Regarding S3, there was a significant difference in 24h compared to Control-Oh for both solutions, and a significant difference between FBP-6h/FBP-24h. Regarding S4, there was a difference between Control-Oh/HTK-24h and FBP-24h/HTK-24h, higher in HTK. There was a difference between Control-Oh/FBP-24h for Swelling, higher in FBP. Conclusion: Fructose-1,6-Bisphosphate showed better performance at nitrate and aspartate aminotransferase compared to histidine-tryptophan-ketoglutarate.

引用

页码：1 / 8

页数：8

共 25 条

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