Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis

被引：8
|
作者：
Zavala-Flores, Laura M. [2 ]
Villatoro-Hernandez, Julio [2 ]
Gamez-Escobedo, Anali [3 ]
Franco-Molina, Moises [3 ]
Rangel-Colmenero, Blanca R. [2 ]
Villanueva-Olivo, Arnulfo [2 ]
Gutierrez-Puente, Yolanda [4 ]
Montes de Oca-Luna, Roberto [2 ]
Valdes-Flores, Jesus [5 ]
Saucedo-Cardenas, Odila [1 ,2 ]
机构：
[1] IMSS, Ctr Invest Biomed Noreste, Div Genet, Monterrey 64720, NL, Mexico
[2] UANL, Fac Med, Dept Histol, Monterrey, NL, Mexico
[3] UANL, Dept Microbiol & Immunol, San Nicolas De Los Garza, NL, Mexico
[4] UANL, Dept Bioquim, Fac Ciencias Biol, San Nicolas De Los Garza, NL, Mexico
[5] CINVESTAV, Dept Bioquim, Mexico City 07360, DF, Mexico
来源：
BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 02期
关键词：
Biological activity; Lactococcus lactis; Lymphotactin; Secretion; CD8(+) T-CELLS; MUCOSAL DELIVERY; ACID BACTERIA; EXPRESSION;
D O I：
10.1007/s10529-008-9855-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Lymphotactin-XCL1 is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells. We expressed human lymphotactin (LTN) by the lactic-acid bacterium Lactococcus lactis. Biological activity of LTN was confirmed by chemo-attraction of human T-cells by chemotaxis demonstrating, for the first time, how this chemokine secreted by a food-grade prokaryote retains biological activity and chemoattracts T lymphocytes. This strain thus represents a feasible well-tolerated vector to deliver active LTN at a mucosal level.
引用
收藏
页码：215 / 220
页数：6
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