Down-regulation of miRNA-320c promotes tumor growth and metastasis and predicts poor prognosis in human glioma

被引：14
作者：
Lv, Qiao-Li [1 ]
Zhu, Hui-Ting [2 ]
Li, Hong-Mi [3 ]
Cheng, Xiao-Hua [4 ]
Zhou, Hong-Hao [5 ]
Chen, Shu-Hui [3 ]
机构：
[1] Jiangxi Canc Hosp, Dept Sci & Educ, Jiangxi Key Lab Translat Canc Res, Nanchang 330029, Jiangxi, Peoples R China
[2] Jiangxi Prov Childrens Hosp, Dept Pharm, Nanchang 330029, Jiangxi, Peoples R China
[3] Jiangxi Canc Hosp, Dept Radiat Oncol, 519 Beijing East Rd, Nanchang 330029, Jiangxi, Peoples R China
[4] Nanchang Univ, Dept Pharm, Affiliated Hosp 1, Nanchang 330029, Jiangxi, Peoples R China
[5] Cent S Univ, Dept Clin Pharmacol, Xiangya Hosp, 110 Xiang Ya Rd, Changsha 410008, Hunan, Peoples R China
来源：
BRAIN RESEARCH BULLETIN | 2018年 / 139卷
关键词：
miRNA-320c; Glioma; Prognosis biomarker; CELL-GROWTH; ACTIVATED MICRORNA; CANCER; EXPRESSION; PROLIFERATION; CISPLATIN; CHEMOSENSITIVITY; ANGIOGENESIS; RESISTANCE; MIR-9;
D O I：
10.1016/j.brainresbull.2018.02.009
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Emerging studies show that dysregulated miRNAs are implicated in tumorigenesis and progression of various cancers. MiRNA-320c, an important member of miRNA-320 family, was characterized as a new candidate miRNA that suppressed the development of colorectal cancer and bladder cancer. However, the function of miRNA-320c in human glioma remained unclear. Here, we found that miRNA-320c was significantly down regulated in glioma tissues in contrast with normal brain tissues, being tightly related to clinical stage of glioma by qRT-PCR. Moreover, Kaplan-Meier analysis demonstrated that patients with low miRNA-320c expression had a shorter survival. Multivariate Cox regression analysis indicated that miRNA-320c could serve as an independent poor prognostic factor for patients with glioma. Functionally, overexpression of miRNA-320c could dramatically inhibit glioma cell proliferation, migration and invasion, as well as promote apoptosis. Further analysis indicated that overexpression of miRNA-320c dramatically led to the G0/G1 phase arrest and correspondingly decreased the percentage of S phase cells by suppressing the expression of G1/S transition key regulators, such as Cyclin D1 and CDK6. Additionally, up-regulation of miRNA-320c could significantly impair migration and invasion of glioma cells via reducing the expression of MMP2, MMP9, N-cadherin and Integrin beta 1. Collectively, our data revealed that miRNA-320c played a crucial role in the carcinoma processes of glioma and might serve as a new prognosis biomarker and therapeutic target of glioma.
引用
收藏
页码：125 / 132
页数：8
相关论文
共 31 条
[1]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[2]   Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo [J].
Chen, Hua ;
Huang, Qiang ;
Dong, Jun ;
Zhai, De-Zhong ;
Wang, Ai-Dong ;
Lan, Qing .
BMC CANCER, 2008, 8 (1)
[3]   MicroRNA-370 suppresses proliferation and promotes endometrioid ovarian cancer chemosensitivity to cDDP by negatively regulating ENG [J].
Chen, Xiao-Ping ;
Chen, You-Guo ;
Lan, Jian-Yun ;
Shen, Zong-Ji .
CANCER LETTERS, 2014, 353 (02) :201-210
[4]   Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients [J].
Delic, Denis ;
Eisele, Claudia ;
Schmid, Ramona ;
Baum, Patrick ;
Wiech, Franziska ;
Gerl, Martin ;
Zimdahl, Heike ;
Pullen, Steven S. ;
Urquhart, Richard .
PLOS ONE, 2016, 11 (03)
[5]   Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster [J].
Dews, Michael ;
Homayouni, Asal ;
Yu, Duonan ;
Murphy, Danielle ;
Sevignani, Cinzia ;
Wentzel, Erik ;
Furth, Emma E. ;
Lee, William M. ;
Enders, Greg H. ;
T Mendell, Joshua ;
Thomas-Tikhonenko, Andrei .
NATURE GENETICS, 2006, 38 (09) :1060-1065
[6]   MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation [J].
Felli, N ;
Fontana, L ;
Pelosi, E ;
Botta, R ;
Bonci, D ;
Facchiano, F ;
Liuzzi, F ;
Lulli, V ;
Morsilli, O ;
Santoro, S ;
Valtieri, M ;
Calin, GA ;
Liu, CG ;
Sorrentino, A ;
Croce, CM ;
Peschle, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (50) :18081-18086
[7]   Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? [J].
Filipowicz, Witold ;
Bhattacharyya, Suvendra N. ;
Sonenberg, Nahum .
NATURE REVIEWS GENETICS, 2008, 9 (02) :102-114
[8]   microRNA-320/RUNX2 axis regulates adipocytic differentiation of human mesenchymal (skeletal) stem cells [J].
Hamam, D. ;
Ali, D. ;
Vishnubalaji, R. ;
Hamam, R. ;
Al-Nbaheen, M. ;
Chen, L. ;
Kassem, M. ;
Aldahmash, A. ;
Alajez, N. M. .
CELL DEATH & DISEASE, 2014, 5 :e1499-e1499
[9]   MicroRNAs: Predictors and modifiers of chemo- and radiotherapy in different tumour types [J].
Hummel, Richard ;
Hussey, Damian J. ;
Haier, Joery .
EUROPEAN JOURNAL OF CANCER, 2010, 46 (02) :298-311
[10]   miR-320c regulates gemcitabine-resistance in pancreatic cancer via SMARCC1 [J].
Iwagami, Y. ;
Eguchi, H. ;
Nagano, H. ;
Akita, H. ;
Hama, N. ;
Wada, H. ;
Kawamoto, K. ;
Kobayashi, S. ;
Tomokuni, A. ;
Tomimaru, Y. ;
Mori, M. ;
Doki, Y. .
BRITISH JOURNAL OF CANCER, 2013, 109 (02) :502-511
1234