Molecular Detection and Identification of a 16SrVI Group Phytoplasma Associated with Tomato Big Bud Disease in Xinjiang, China

被引：4

作者：

Du, Yejuan ^{[1
]}

Mou, Haiqing ^{[2
]}

Shi, Baoping ^{[1
]}

Xu, Xia ^{[2
]}

Xiang, Benchun ^{[1
]}

机构：

[1] Shihezi Univ, Coll Agr, Key Lab Univ Xinjiang Uygur Autonomous Reg Oasis, Shihezi, Peoples R China

[2] Chinese Acad Inspect & Quarantine, Inst Plant Quarantine, Beijing 100029, Peoples R China

来源：

JOURNAL OF PHYTOPATHOLOGY | 2013年 / 161卷 / 11-12期

关键词：

big bud; Candidatus Phytoplasma trifolii; China; tomato; virtual RFLP; CLOVER PROLIFERATION PHYTOPLASMA; SOLANACEOUS CROPS; TRIFOLII;

D O I：

10.1111/jph.12136

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In 2010, tomato plants with big bud symptoms were observed in Xinjiang, China. PCR products of approximately 1.2 and 2.8kb were amplified from infected tomato tissues but not from asymptomatic plants. A comparison of 16S rDNA sequences showed that the casual tomato big bud (TBB) phytoplasma was closely (99%) related to the Candidatus Phytoplasma trifolii' (16SrVI group). The TBB phytoplasma clustered into one branch with the Loofah witches'-broom phytoplasma according to the 23S rDNA analysis but with no other member of the 16SrVI group. The cause of TBB symptoms was identified as Ca. Phytoplasma trifolii' (16SrVI group) by PCR, virtual RFLP and sequencing analyses. This is the first report of a phytoplasma related to Ca. Phytoplasma trifolii' causing TBB disease in China.

引用

页码：870 / 873

页数：4

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