Surface-Coated Probe Nanoelectrospray Ionization Mass Spectrometry for Analysis of Target Compounds in Individual Small Organisms

被引:66
作者
Deng, Jiewei [1 ]
Yang, Yunyun [2 ,3 ]
Xu, Mingzhi [1 ]
Wang, Xiaowei [4 ]
Lin, Li [1 ]
Yao, Zhong-Ping [5 ,6 ]
Luan, Tiangang [1 ]
机构
[1] Sun Yat Sen Univ, MOE Key Lab Aquat Prod Safety, Sch Life Sci, South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China
[2] China Natl Analyt Ctr Guangzhou, Guangdong Prov Key Lab Emergency Test Dangerous C, Guangzhou 510070, Guangdong, Peoples R China
[3] China Natl Analyt Ctr Guangzhou, Guangdong Prov Publ Lab Anal & Testing Technol, Guangzhou 510070, Guangdong, Peoples R China
[4] Sun Yat Sen Univ, Sch Marine Sci, Guangdong Prov Key Lab Marine Resources & Coastal, Guangzhou 510275, Guangdong, Peoples R China
[5] Hong Kong Polytech Univ, State Key Lab Chirosci, Hong Kong, Hong Kong, Peoples R China
[6] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hong Kong, Hong Kong, Peoples R China
基金
中国国家自然科学基金;
关键词
SOLID-PHASE MICROEXTRACTION; SINGLE CELLS; ELECTROSPRAY-IONIZATION; DAPHNIA-MAGNA; PERFLUOROOCTANOIC ACID; LIVING CELLS; METABOLITES; SAMPLES; WATER; BIOACCUMULATION;
D O I
10.1021/acs.analchem.5b03110
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Analysis of target compounds in individual small organisms is of significant importance for biological, environmental, medicinal, and toxicological investigation. In this study, we reported the development of a novel solid-phase microextraction (SPME) based ambient mass spectrometry (MS) method named surface-coated probe nanoelectrospray ionization (SCP-nanoESI)-MS for analysis of target compounds in individual small organisms with sizes at micrometer-to-millimeter level. SCP-nanoESI-MS analysis involves three procedures: (1) modification of adsorbent at the surface of a fine metal probe to form a specially designed surface-coated SPME probe with probe-end diameter at several-micrometer level, (2) application of the surface-coated SPME probe for enrichment of target analytes from individual small organisms, and (3) employment of a nanospray tip and some solvent to desorb the analytes and induce nanoESI for mass spectrometric analysis under ambient condition. A SCP-nanoESI-MS method for determination of the perfluorinated compounds (PFCs) in individual Daphnia magna was developed. The method showed satisfactory linearities for analysis of real Daphnia magna samples, with correlation coefficient values (R-2) of 0.9984 and 0.9956 for perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), respectively. The limits of detection were 0.02 and 0.03 ng/mL for PFOS and PFOA, respectively. By using the proposed method, the amount, bioaccumulation kinetics, and distribution of PFOS and PFOA in individual Daphnia magna were successfully investigated.
引用
收藏
页码:9923 / 9930
页数:8
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