A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes

被引：3

作者：

Bai, Chunsen ^{[1
]}

Klimkowski, Piotr ^{[1
]}

Jin, Cheng ^{[1
]}

Kuchlyan, Jagannath ^{[1
]}

El-Sagheer, Afaf H. ^{[1
,2
]}

Brown, Tom ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, 12 Mansfield Rd, Oxford OX1 3TA, England

[2] Suez Univ, Fac Petr & Min Engn, Dept Sci & Math, Chem Branch, Suez 43721, Egypt

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2022年 / 20卷 / 44期

关键词：

DYE THIAZOLE ORANGE; HYBEACON(TM) PROBES; FIT-PROBES; DNA; HYBRIDIZATION;

D O I：

10.1039/d2ob01899c

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Oligonucleotides labelled with thiazole orange intercalator and a reporter dye on the same thymine base have been synthesized. The key phosphoramidite (AP-C3 dT) contains an alkyne and amine, enabling dual orthogonal labelling of the nucleobase. Multiple monomers can be added to produce heavily functionalised oligonucleotides. In their DNA and 2 '-OMe RNA formats these combination probes display high duplex stability and fluorescence when bound to complementary DNA and RNA.

引用

页码：8618 / 8622

页数：5

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