Aging decreases antioxidant effects and increases lipid peroxidation in the Apolipoprotein E deficient mouse

被引：19

作者：

Honma, Taro ^{[1
]}

Tsuduki, Tsuyoshi ^{[1
]}

Sugawara, Soko ^{[1
]}

Kitano, Yasuna ^{[1
]}

Ito, Junya ^{[1
]}

Kijima, Ryo ^{[1
]}

Tsubata, Mari ^{[1
]}

Nakagawa, Kiyotaka ^{[2
]}

Miyazawa, Teruo ^{[2
]}

机构：

[1] Tohoku Univ, Grad Sch Agr, Lab Food & Biomol Sci, Sendai, Miyagi 9818555, Japan

[2] Tohoku Univ, Grad Sch Agr, Food & Biodynam Chem Lab, Sendai, Miyagi 9818555, Japan

来源：

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION | 2013年 / 52卷 / 03期

基金：

日本学术振兴会;

关键词：

aging; apolipoprotein E; lipid peroxidation; senescence; TBARS; CONJUGATED TRIENE SYSTEM; LINOLENIC ACID ISOMER; HIGH-DENSITY-LIPOPROTEIN; ALPHA-ELEOSTEARIC-ACID; ADIPOSE-TISSUE; SAMP10; MOUSE; BLOOD-PLASMA; LIFE-SPAN; IN-VITRO; HIGH-FAT;

D O I：

10.3164/jcbn.12-85

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

In this study, to study the effect of aging and Apolipoprotein E (ApoE) deficiency on antioxidant ability in mice, we examined whether lipid peroxidation is promoted by aging in ApoE deficient (ApoE(-/-)) mice, which have a shorter lifespan than normal mice. The levels of thiobarbituric acid-reactive substances (TBARS), a biomarker of lipid peroxidation, were measured in plasma and liver in ApoE(-/-) mice aged 12 weeks (young) and 52 weeks (early stage of senescence). TBARS in plasma and liver were significantly increased by aging. Next, we examined the reasons why lipid peroxidation was promoted by aging, based on measurement of protein and mRNA levels for antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in liver in ApoE(-/-) mice aged 12 and 52 weeks. The levels of superoxide dismutase 1 and 2 in liver were significantly decreased by aging. The mRNA level of catalase was also significantly decreased and the mRNA levels of superoxide dismutase 1, superoxide dismutase 2 and glutathione peroxidase 1 all showed a tendency to decrease with age. These results suggest that lipid peroxidation is caused by reduction of antioxidant activity with aging and that this promotes senescence and shortens lifespan in ApoE(-/-) mice.

引用

页码：234 / 240

页数：7

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