In the present study, the effect of supplementation of vitamin E in ochratoxin A (OTA) contaminated diet in ameliorating adverse effects of ochratoxin on biochemical parameters and immune response in broiler chickens was investigated. Day old broiler chicks (240) were divided into 6 treatment groups (T1-control (basal diet), T2 - T1 + 200 ppb OTA, T3 -T1 + 100 mg vit. E, T4 -T1 + 200 mg vit. E, T5 -T2 + 100 mg vit. E and T6 -T2 + 200 mg vit. E). Each diet was fed to 5 replicated groups of 8 birds each from 0 to 42 days of age. The blood samples were collected on 42 days of age of broilers. The total serum protein and haemoglobin (Hb) concentrations in T2 (200 ppb OTA) and T5 (200 ppb OTA + 100 mg vit. E) were lower (P<0.05) than that of control (T1) diet. The serum protein and Hb contents in group T6 was higher (P<0.05) than that of T2 but statistically similar to that of control. The serum uric acid, creatinine, alkaline phosphatase (ALP), serum glutamic oxaloacetic transferase (SGOT) and serum glutamic pyruvic transferase (SGPT) value in T2 was higher (P<0.05) than that of T1. The serum uric acid, creatinine, ALP, SGOT and SGPT value in T6 (200 ppb OTA + 200 mg vit. E) was lower (P<0.05) than that of T2 and statistically similar to that of control (T1). The CMI and HA titre value in T2 was lower (P<0.05) than that of control (T1). The CMI and HA titre value of T3 and T4 was statistically similar to that of control. The CMI value of T5 and T6 was lower (P<0.05) than control, but higher (P<0.05) than T2. The CMI of group T6 was higher (P<0.05) than T5. The HA titre value of group T5 was higher (P<0.05) than T2 and lower than T1. The HA titre value in T6 was statistically similar to that of T1, indicating that inclusion of vit. E (200 mg/kg diet) ameliorated the adverse effect of ochratoxicosis in broiler chickens. Thus, it was concluded that ochratoxin contamination at 200 ppb level in broiler diet had adverse effect on blood biochemical profile and immune response, which were restored through supplementation of vitamin E at 200 mg/kg of feed in ochratoxin contaminated diet in broiler chickens.
