Propolis nanoparticles modulate the inflammatory and apoptotic pathways in carbon tetrachloride-induced liver fibrosis and nephropathy in rats

This study assessed the use of emulsion-produced propolis nanoparticles for treating carbon tetrachloride (CCl4)-induced liver fibrosis and nephropathy on albino rat model. The evaluation of hepatotoxicity, nephrotoxicity, and the treatment outcomes involved biochemical investigations of blood samples as well as molecular analysis, and histopathological assessment for liver and kidney tissue samples. CCl(4)treatment caused elevated biochemical indicators of hepatotoxic and nephrotoxic effects as detected by liver and kidney functions tests, which improved gradually with propolis nanoparticles treatment. The molecular studies showed an increase in transforming growth factor beta (TGF-beta), Nephrin, and Caspase-9, while Bcl-2 levels dropped in both liver and kidney tissue samples; such changes were normalized after treatment. The histological findings confirm both biochemical and molecular studies. Our results indicated that propolis nanoparticles had an anti-inflammatory effect as proved by decreased expression of TGF-beta in liver tissue and Nephrin in kidney tissue. The propolis nanoparticles showed an anti-apoptotic effect on liver and kidney tissue increasing the expression of Bcl-2 and decreasing the expression of Caspase-9.