Prevalence and clinical relevance of Th17 cells in patients with gastric cancer

被引:65
作者
Yamada, Yoshinori [1 ]
Saito, Hiroaki [1 ]
Ikeguchi, Masahide [1 ]
机构
[1] Tottori Univ, Sch Med, Dept Surg, Div Surg Oncol, Yonago, Tottori 6838504, Japan
关键词
Gastric cancer; Interleukin-17; Th17; T-CELL; PROMOTES ANGIOGENESIS; OVARIAN-CANCER; TUMOR-GROWTH; IL-17; CYTOKINE; HELPER; LYMPHOCYTES; EXPRESSION; RECEPTOR;
D O I
10.1016/j.jss.2012.07.055
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: Th17 cells have recently been identified as a distinct T helper (Th) lineage in a cancer animal model and in human cancers. Their specific role in tumor immunity is unclear. We, therefore, sought to evaluate the role of Th17 cells in gastric cancer. Methods: The prevalence of Th1, Th2, Treg, and Th17 cells in both peripheral blood mononuclear cells (PBMC) and gastric tissue was evaluated by multicolor flow cytometry. The concentration of interleukin (IL)-17 in sera was quantitated by enzyme-linked immunosorbent assay. Results: We observed a clear difference in the prevalence of Th17 cells in PBMC (0.34 +/- 0.24%) versus gastric cancer tissues (19.4 +/- 12.1) (P = 0.0002). Subset-specific phenotypic analysis of CD4+ T cells in both PBMC and gastric cancer tissue showed that Th1 and Treg cells predominate in PBMC, whereas Th17 cells are the most abundant CD4+ T cell subset in cancerous tissue. The concentrations of IL-17, a hallmark of Th17, in gastric cancer patients and normal controls were 0.6 +/- 0.67 and 0.16 +/- 0.19 pg/mL (P = 0.0032). Five-year survival rates of patients with high IL-17 and low IL-17 concentration were 47.1% and 83.9% (P = 0.0075). Multivariate analysis indicated that IL-17 concentration was an independent prognostic indicator, as well as lymph node metastasis. Conclusions: There was a significant skewing toward a Th17 phenotype in gastric cancer tissue. IL-17 seems to play an important role in the progression of gastric cancer. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:685 / 691
页数:7
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