Indirect sandwich ELISA for antigen detection of African swine fever virus: Comparison of polyclonal and monoclonal antibodies

被引：31
作者：
Hutchings, GH [1 ]
Ferris, NP [1 ]
机构：
[1] Inst Anim Hlth, Pirbright Lab, Woking GU24 0NF, Surrey, England
来源：
JOURNAL OF VIROLOGICAL METHODS | 2006年 / 131卷 / 02期
关键词：
African swine fever; diagnosis; ELISA; antigen detection; polyclonal and monoclonal antibodies;
D O I：
10.1016/j.jviromet.2005.08.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two indirect, sandwich ELISAs are described for use in African swine fever (ASF) diagnosis. One assay uses polyclonal serum raised in rabbits and guinea pigs against the cytoplasmic soluble ASF virus protein and the second. a combination of monoclonal antibody raised against the VP73 protein and rabbit polyclonal serum. Both assays have been shown to detect antigen of representative field strains of phylogenetically distinct groupings of ASIF virus but the ELISA, which utilises polyclonal antisera was slightly more sensitive than that using the monoclonal antibody. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：213 / 217
页数：5
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