Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae)

被引：16

作者：

Zheng, J. Y. ^{[1
]}

Wang, H. ^{[1
]}

Chen, X. X. ^{[1
]}

Wang, P. ^{[1
]}

Gao, P. ^{[1
]}

Li, X. N. ^{[2
]}

Zhu, G. P. ^{[1
]}

机构：

[1] Anhui Normal Univ, Key Lab Mol Evolut & Biodivers, Inst Mol Biol & Biotechnol, Wuhu, Peoples R China

[2] Yijishan Hosp, Wannan Med Coll, Wuhu, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 03期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

Paris polyphylla var. chinensis; Microsatellite; Molecular marker; Population genetics; EFFICIENCY; RAPD; SSR;

D O I：

10.4238/2012.August.6.1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellite loci from a (CT)(n)-enriched genomic library of P. polyphylla var. chinensis. The polymorphism at each locus was analyzed by screening 30 individuals from a natural population. The number of alleles ranged from 2 to 5. The observed and expected heterozygosities ranged from 0.000 to 0.467, with a mean of 0.247, and from 0.383 to 0.662 with a mean of 0.537, respectively. Six loci (Pp1, Pp3, Pp6, Pp7, Pp9, and Pp12) were found to significantly deviate from Hardy-Weinberg equilibrium. This may be due to the small population size, inbreeding or null alleles. Five of the pairwise comparisons (Pp1 and Pp4, Pp2 and Pp5, Pp2 and Pp9, Pp2 and Pp12, Pp11 and Pp12) exhibited significant linkage disequilibrium (P < 0.05). We conclude that these microsatellite markers will be useful for population genetic studies of P. polyphylla var. chinensis.

引用

页码：1975 / 1980

页数：6

共 22 条

[1]

Belkhir KP., 2001, GENETIX LOGICIEL WIN

[2]

Bloor P.A., 2001, Microsatellite Libraries by Enrichment

[3] Genetic diversity in local and commercial dry bean (Phaseolus vulgaris) accessions based on microsatellite markers [J].

Cabral, P. D. S. ;

Soares, T. C. B. ;

Lima, A. B. P. ;

de Miranda, F. D. ;

Souza, F. B. ;

Goncalves, L. S. A. .

GENETICS AND MOLECULAR RESEARCH, 2011, 10 (01) :140-149

[4] Relative mutation rates at di-, tri-, and tetranucleotide microsatellite loci [J].

Chakraborty, R ;

Kimmel, M ;

Stivers, DN ;

Davison, LJ ;

Deka, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (03) :1041-1046

[5] Genetic diversity of eggplant (Solanum melongena) germplasm from Turkey assessed by SSR and RAPD markers [J].

Demir, K. ;

Bakir, M. ;

Sarikamis, G. ;

Acunalp, S. .

GENETICS AND MOLECULAR RESEARCH, 2010, 9 (03) :1568-1576

[6]

Doyle JJ, 1987, PHYTOCHEM B, V19, P11, DOI DOI 10.2307/4119796

[7] Microsatellites: Simple sequences with complex evolution [J].

Ellegren, H .

NATURE REVIEWS GENETICS, 2004, 5 (06) :435-445

[8] Heirloom tomato gene bank: assessing genetic divergence based on morphological, agronomic and molecular data using a Ward-modified location model [J].

Goncalves, L. S. A. ;

Rodrigues, R. ;

do Amaral Junior, A. T. ;

Karasawa, M. ;

Sudre, C. P. .

GENETICS AND MOLECULAR RESEARCH, 2009, 8 (01) :364-374

[9]

Ji S., 2001, PREPARED CHINESE MED, V23, P212

[10] Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines [J].

Leal, A. A. ;

Mangolin, C. A. ;

do Amaral Junior, A. T. ;

Goncalves, L. S. A. ;

Scapim, C. A. ;

Mott, A. S. ;

Eloi, I. B. O. ;

Cordoves, V. ;

da Silva, M. F. P. .

GENETICS AND MOLECULAR RESEARCH, 2010, 9 (01) :9-18

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