Development of HPTLC method for the estimation of Metoprolol succinate in bulk and in tablet dosage form

A simple, rapid, reliable and accurate HPTLC method has been developed for the quantitative determination of metoprolol succinate in bulk and in tablets. The drug was extracted from ( Met-XL* 50). Various aliquots of this sample solution were spotted automatically by means of Camag ( Muttenz, Switzerland) Linomat V applicator on Merck HPTLC plates ( 0.2 mm thickness) precoated with silica gel60 F-254 on aluminium sheet as stationary phase prewashed with methanol using toluene-methanol-triethylamine 3:0.5:0.3(v/v/v) as mobile phase. The spots were scanned at lambda = 274 nm using Camag TLC scanner3. The R-f value of metoprolol succinate was 0.40. Calibration curves were linear in the range 5000-10000 ng per spot. The limit of detection and quantitation for metoprolol succinate were found to be 431.22 ng per spot and 1306.74 ng per spot respectively. The suitability of this method for the quantitative determination of compound was proved by validation in accordance with requirements of pharmaceutical regulatory standards. The proposed method is valid, simple, sensitive and accurate. Therefore this method was applied for routine analysis of these drugs in bulk and formulation.