Biochemical, physiological, and molecular characterization of Dickeya dianthicola (formerly named Erwinia chrysanthemi) causing potato blackleg disease in Japan

被引：14

作者：

Fujimoto, Taketo ^{[1
]}

Yasuoka, Shinji ^{[2
]}

Aono, Yoshiyuki ^{[3
]}

Nakayama, Takato ^{[1
]}

Ohki, Takehiro ^{[1
]}

Sayama, Mitsuru ^{[1
]}

Maoka, Tetsuo ^{[1
]}

机构：

[1] Natl Agr & Food Res Org HARC NARO, Hokkaido Agr Res Ctr, Toyohira Ku, Sapporo, Hokkaido 0628555, Japan

[2] Hokkaido Res Org, Tokachi Agr Expt Stn, Memuro, Hokkaido 0820081, Japan

[3] NARO, Hokkaido Chuo Stn, Ctr Seeds & Seedlings, Kitahiroshima, Hokkaido 0611102, Japan

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2018年 / 84卷 / 02期

关键词：

Blackleg disease; Dickeya dianthicola; Solanum tuberosum; Bacterial wilt; PECTOBACTERIUM-CAROTOVORUM; SOFT-ROT; SP-NOV; STRAINS; SPP; IDENTIFICATION; FRAGMENTS; SEQUENCES; SYSTEM; CLADE;

D O I：

10.1007/s10327-018-0772-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The taxonomic assignment of Japanese potato blackleg isolates of Dickeya spp. has not been confirmed after the changes in their former name, Erwinia chrysanthemi. Therefore, we investigated and identified 23 representative isolates of Dickeya spp. from symptomatic stems of potatoes in Japan, with biochemical tests and phylogenetic sequence analysis using recA, dnaX, rpoD, gyrB, and 16S rDNA sequences. Results of our biochemical tests showed that all isolates can be assigned to phenon 5 and biovar 1, which are associated with D. dianthicola. Based on the recA, dnaX, rpoD, gyrB, and 16S rDNA sequences, all isolates are in the same clade with D. dianthicola and were clearly distinguished from D. chrysanthemi, D. dadantii, D. dadantii subsp. dieffenbachiae, D. solani, D. zeae, and D. paradisiaca. Therefore, we conclude that Dickeya spp. isolated from potatoes with blackleg symptoms in Japan are D. dianthicola.

引用

页码：124 / 136

页数：13

共 3 条

[1] Biochemical, physiological, and molecular characterization of Dickeya dianthicola (formerly named Erwinia chrysanthemi) causing potato blackleg disease in Japan
Taketo Fujimoto
Shinji Yasuoka
Yoshiyuki Aono
Takato Nakayama
Takehiro Ohki
Mitsuru Sayama
Tetsuo Maoka
Journal of General Plant Pathology, 2018, 84 : 124 - 136
[2] First Report of Dickeya dianthicola Causing Blackleg Disease on Potato in Morocco
Oulghazi, S.
Khayi, S.
Lafkih, N.
Massaoudi, Y.
El Karkouri, A.
El Hassouni, M.
Faure, D.
Moumni, M.
PLANT DISEASE, 2017, 101 (09) : 1671 - 1672
[3] First Report of Dickeya dianthicola Causing Blackleg Disease on Potato Plants in Pakistan
Sarfraz, S.
Riaz, K.
Oulghazi, S.
Cigna, J.
Alam, M. W.
Dessaux, Y.
Faure, D.
PLANT DISEASE, 2018, 102 (10) : 2027 - 2028

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