Trans-splicing in C. elegans generates the negative RNAi regulator ERI-6/7

被引：80
作者：
Fischer, Sylvia E. J. [1 ,2 ]
Butler, Maurice D. [1 ,2 ]
Pan, Qi [1 ,2 ]
Ruvkun, Gary [1 ,2 ]
机构：
[1] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA
来源：
NATURE | 2008年 / 455卷 / 7212期
关键词：
D O I：
10.1038/nature07274
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Mutations that enhance the response to double- stranded RNA ( dsRNA) have revealed components of the RNA interference ( RNAi) pathway or related small RNA pathways. To explore these small RNA pathways, we screened for Caenorhabditis elegans mutants displaying an enhanced response to exogenous dsRNAs. Here we describe the isolation of mutations in two adjacent, divergently transcribed open reading frames ( eri-6 and eri-7) that fail to complement. eri-6 and eri- 7 produce separate pre- messenger RNAs ( pre- mRNAs) that are trans- spliced to form a functional mRNA, eri- 6/ 7. Trans- splicing of eri6/7 is mediated by a direct repeat that flanks the eri- 6 gene. Adenosine to inosine editing within untranslated regions of eri- 6 and eri- 7 pre- mRNAs reveals a double- stranded pre- mRNA intermediate, forming in the nucleus before splicing occurs. The ERI-6/7 protein is a superfamily I helicase that both negatively regulates the exogenous RNAi pathway and functions in an endogenous RNAi pathway.
引用
收藏
页码：491 / U23
页数：7
相关论文
共 43 条
  • [1] Gene silencing in Caenorhabditis elegans by transitive RNA interference
    Alder, MN
    Dames, S
    Gaudet, J
    Mango, SE
    [J]. RNA, 2003, 9 (01) : 25 - 32
  • [2] RNA editing by adenosine deaminases that act on RNA
    Bass, BL
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 2002, 71 : 817 - 846
  • [3] The Caenorhabditis elegans gene lin-1 encodes an ETS-domain protein and defines a branch of the vulval induction pathway
    Beitel, GJ
    Tuck, S
    Greenwald, I
    Horvitz, HR
    [J]. GENES & DEVELOPMENT, 1995, 9 (24) : 3149 - 3162
  • [4] Role for a bidentate ribonuclease in the initiation step of RNA interference
    Bernstein, E
    Caudy, AA
    Hammond, SM
    Hannon, GJ
    [J]. NATURE, 2001, 409 (6818) : 363 - 366
  • [5] Rescue of polyglutamine-mediated cytotoxicity by double-stranded RNA-mediated RNA interference
    Caplen, NJ
    Taylor, JP
    Statham, VS
    Tanaka, F
    Fire, A
    Morgan, RA
    [J]. HUMAN MOLECULAR GENETICS, 2002, 11 (02) : 175 - 184
  • [6] A putative catenin-cadherin system mediates morphogenesis of the Caenorhabditis elegans embryo
    Costa, M
    Raich, W
    Agbunag, C
    Leung, B
    Hardin, J
    Priess, JR
    [J]. JOURNAL OF CELL BIOLOGY, 1998, 141 (01) : 297 - 308
  • [7] SDE3 encodes an RNA helicase required for posttranscriptional gene silencing in Arabidopsis
    Dalmay, T
    Horsefield, R
    Braunstein, TH
    Baulcombe, DC
    [J]. EMBO JOURNAL, 2001, 20 (08) : 2069 - 2077
  • [8] Transgene analysis proves mRNA trans-splicing at the complex mod(mdg4) locus Drosophila
    Dorn, R
    Reuter, G
    Loewendorf, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) : 9724 - 9729
  • [9] Duchaine TF, 2006, CELL, V124, P343, DOI [10.1016/j.cell.2005.11.036, 10.1016/j.cel.2005.11.036]
  • [10] Genomes:: A helpful cousin for our favourite worm
    Félix, MA
    [J]. CURRENT BIOLOGY, 2004, 14 (02) : R75 - R77
12345