Hyperbaric oxygen treatment and N-acetylcysteine ameliorate acetaminophen-induced liver injury in a rat model

被引:12
作者
Taslipinar, M. Y. [1 ]
Aydin, I. [2 ]
Kaldirim, U. [3 ]
Aydin, F. N. [2 ]
Agilli, M. [2 ]
Eyi, Y. E. [3 ]
Tuncer, S. K. [3 ]
Altayli, E. [4 ]
Ucar, F. [1 ]
Macit, E. [5 ]
Toygar, M. [6 ]
Yigit, N. [7 ]
Cayci, T. [2 ]
机构
[1] Diskapi Yildirim Beyazit Training & Res Hosp, Dept Biochem, TR-06110 Ankara, Turkey
[2] Gulhane Mil Med Acad, Dept Clin Biochem, Ankara, Turkey
[3] Gulhane Mil Med Acad, Dept Emergency Med, Ankara, Turkey
[4] Gulhane Mil Med Acad, Dept Med Biol, Ankara, Turkey
[5] Gulhane Mil Med Acad, Dept Toxicol, Ankara, Turkey
[6] Gulhane Mil Med Acad, Dept Forens Med, Ankara, Turkey
[7] Gulhane Mil Med Acad, Dept Pathol, Ankara, Turkey
关键词
Acetaminophen; N-acetylcysteine; hyperbaric oxygen; inflammation; liver injury; OXIDATIVE STRESS; OZONE THERAPY; SEPTIC RATS; HEPATOTOXICITY; INFLAMMATION; EXPRESSION; NEOPTERIN; SEVERITY; MICE; MACROPHAGES;
D O I
10.1177/0960327113499167
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
An overdose of acetaminophen (APAP) produces centrilobular hepatocellular necrosis. We aimed to investigate the hepatoprotective effects of N-acetylcysteine (NAC) only and hyperbaric oxygen (O-2) treatment (HBOT) combined with NAC, and their anti-inflammatory properties in liver tissue. In the current study, a total of 32 male Sprague Dawley rats were divided into 4 groups: sham, APAP, NAC, and NAC + HBOT. In the APAP, NAC, and NAC + HBOT groups, liver injury was induced by oral administration of 1 g/kg APAP. The NAC group received 100 mg/kg NAC per day. NAC + HBOT group received intraperitoneal injection of 100 mg/kg/day NAC and were given HBOT at 2.8 ATA pressure with 100% O-2 inhalation for 90 min every 12 h for 5 days. Rats in the sham group received distilled water only by gastric tube. All animals were killed on day 6 after APAP or distilled water administration. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, hepatic neopterin, tumor necrosis factor- (TNF-), and interleukin 6 (IL-6) levels were measured. There was a significant increase in serum AST and ALT activities in the APAP group compared with the sham group (in both p = 0.001). NAC and NAC + HBOT groups had significant decreases in hepatic neopterin, TNF-, and IL-6 levels compared with the APAP group. APAP administration caused extensive hepatic necrosis. NAC and NAC + HBO treatments significantly reduced APAP-induced liver injury. Our results showed that the liver damage in APAP toxicity was attenuated by NAC and NAC + HBO treatments. NAC + HBOT exhibit hepatoprotective activity against APAP-induced liver injury in rats.
引用
收藏
页码:1107 / 1116
页数:10
相关论文
共 44 条
[1]  
Agilli M., 2012, J INVEST BIOCH, V1, P43
[2]   Pressure-related increase of asymmetric dimethylarginine caused by hyperbaric oxygen in the rat brain:: A possible neuroprotective mechanism [J].
Akgul, Emin Ozgur ;
Cakir, Erdinc ;
Ozcan, Omer ;
Yaman, Halil ;
Kurt, Yasemin Gulcan ;
Oter, Sukru ;
Korkmaz, Ahmet ;
Bilgi, Cumhur ;
Erbil, Mehmet Kemal .
NEUROCHEMICAL RESEARCH, 2007, 32 (09) :1586-1591
[3]   Comparison of hyperbaric oxygen and medical ozone therapies in a rat model of experimental distal colitis [J].
Altinel, Ozcan ;
Demirbas, Seref ;
Cakir, Erdinc ;
Yaman, Halil ;
Ozerhan, Ismail H. ;
Duran, Eyup ;
Cayci, Tuncer ;
Akgul, Emin O. ;
Ersoz, Nail ;
Uysal, Bulent ;
Kurt, Bulent ;
Yasar, Mehmet ;
Oter, Sukru ;
Peker, Yusuf .
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2011, 71 (03) :185-192
[4]   Reduced monocyte HLA-DR expression: A novel biomarker of disease severity and outcome in acetaminophen-induced acute liver failure [J].
Antoniades, CG ;
Berry, PA ;
Davies, ET ;
Hussain, M ;
Bernal, W ;
Vergani, D ;
Wendon, J .
HEPATOLOGY, 2006, 44 (01) :34-43
[5]   Persistence of hyperbaric oxygen-induced oxidative effects after exposure in rat brain cortex tissue [J].
Ay, Hakan ;
Topal, Turgut ;
Ozler, Mehmet ;
Uysal, Bulent ;
Korkmaz, Ahmet ;
Oter, Sukru ;
Ogur, Recai ;
Dundar, Kadir .
LIFE SCIENCES, 2007, 80 (22) :2025-2029
[6]   ROLE OF PROINFLAMMATORY CYTOKINES IN ACETAMINOPHEN HEPATOTOXICITY [J].
BLAZKA, ME ;
WILMER, JL ;
HOLLADAY, SD ;
WILSON, RE ;
LUSTER, MI .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1995, 133 (01) :43-52
[7]   The association between neopterin and acetaminophen-induced nephrotoxicity [J].
Cakir, Erdinc ;
Akgul, Ozgur E. ;
Aydin, Ibrahim ;
Cayci, Tuncer ;
Kurt, Yasemin Gulcan ;
Onguru, Onder ;
Aydin, Fevzi N. ;
Agilli, Mehmet ;
Yaman, Halil ;
Ersoz, Nail ;
Bilgic, Serkan ;
Guven, Ahmet ;
Turker, Turker ;
Bilgi, Cumhur ;
Erbil, Kemal M. .
RENAL FAILURE, 2010, 32 (06) :740-746
[8]   Distinct roles of NF-κB p50 in the regulation of acetaminophen-induced inflammatory mediator production and hepatotoxicity [J].
Dambach, DM ;
Durham, SK ;
Laskin, JD ;
Laskin, DL .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2006, 211 (02) :157-165
[9]   Elevated serum neopterirn levels in acetaminophen-induced liver injury [J].
Demirbas, Seref ;
Cakir, Erdinc ;
Akgul, Emin Ozgur ;
Seyrek, Melik ;
Cayci, Tuncer ;
Kurt, Yasemin Gulcan ;
Uysal, Bulent ;
Aydin, Ibrahim ;
Kurt, Bulent ;
Yaman, Halil ;
Erbil, Mehmet Kemal .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2011, 31 (01) :165-170
[10]  
Dodd S, 2008, EXPERT OPIN BIOL TH, V8, P1955, DOI [10.1517/14728220802517901, 10.1517/14728220802517901 ]