Soybean peroxidase-mediated degradation of an azo dye- a detailed mechanistic study

被引:61
作者
Ali, Liaquat [2 ]
Algaithi, Rowdha [2 ]
Habib, Hosam M. [1 ]
Souka, Usama [1 ]
Rauf, Muhammad A. [2 ]
Ashraf, S. Salman [2 ]
机构
[1] UAE Univ, Coll Food & Agr, Dept Nutr & Hlth, Al Ain, U Arab Emirates
[2] UAE Univ, Coll Sci, Dept Chem, Al Ain, U Arab Emirates
关键词
Azo dye; Degradation; Enzyme; Mediator; LC/MS; Metabolites; PHOTOCATALYTIC DEGRADATION; ORANGE G; DECOLORIZATION; OXIDATION; REMOVAL; PRODUCTS; PERFORMANCE; COAGULANT; EFFLUENT; FENTON;
D O I
10.1186/1471-2091-14-35
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Peroxidases are emerging as an important class of enzymes that can be used for the efficient degradation of organic pollutants. However, detailed studies identifying the various intermediates produced and the mechanisms involved in the enzyme-mediated pollutant degradation are not widely published. Results: In the present study, the enzymatic degradation of an azo dye ( Crystal Ponceau 6R, CP6R) was studied using commercially available soybean peroxidase (SBP) enzyme. Several operational parameters affecting the enzymatic degradation of dye were evaluated and optimized, such as initial dye concentration, H2O2 dosage, mediator amount and pH of the solution. Under optimized conditions, 40 ppm dye solution could be completely degraded in under one minute by SBP in the presence of H2O2 and a redox mediator. Dye degradation was also confirmed using HPLC and TOC analyses, which showed that most of the dye was being mineralized to CO2 in the process. Conclusions: Detailed analysis of metabolites, based on LC/MS results, showed that the enzyme-based degradation of the CP6R dye proceeded in two different reaction pathways- via symmetric azo bond cleavage as well as asymmetric azo bond breakage in the dye molecule. In addition, various critical transformative and oxidative steps such as deamination, desulfonation, keto-oxidation are explained on an electronic level. Furthermore, LC/MS/MS analyses confirmed that the end products in both pathways were small chain aliphatic carboxylic acids.
引用
收藏
页数:14
相关论文
共 42 条
[1]   Microbial decolourization of textile waste water [J].
Ali, N. F. ;
El-Mohamedy, R. S. R. .
JOURNAL OF SAUDI CHEMICAL SOCIETY, 2012, 16 (02) :117-123
[2]   A comparative study of Neutral Red decoloration by photo-Fenton and photocatalytic processes [J].
Alnuaimi, Maitha M. ;
Rauf, M. A. ;
Ashraf, S. Salman .
DYES AND PIGMENTS, 2008, 76 (02) :332-337
[3]   Mutagenic and carcinogenic potential of a textile azo dye processing plant effluent that impacts a drinking water source [J].
Alves de Lima, Rodrigo Otavio ;
Bazo, Ana Paula ;
Favero Salvadori, Daisy Maria ;
Rech, Celia Maria ;
Oliveira, Danielle de Palma ;
Umbuzeiro, Gisela de Aragao .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2007, 626 (1-2) :53-60
[4]  
Ateeq H, 2007, DYES PIGMENTS, V75, P395
[5]   Photocatalytic degradation of azo dye in aqueous TiO2 suspension: Reaction pathway and identification of intermediates products by LC/MS [J].
Bansal, Priti ;
Singh, Damanjit ;
Sud, Dhiraj .
SEPARATION AND PURIFICATION TECHNOLOGY, 2010, 72 (03) :357-365
[6]   Comparative study of natural and synthetic phenolic compounds as efficient laccase mediators for the transformation of cationic dye [J].
Bibi, Ismat ;
Bhatti, Haq Nawaz ;
Asgher, Muhammad .
BIOCHEMICAL ENGINEERING JOURNAL, 2011, 56 (03) :225-231
[7]   Aniline mineralization by AOP's: anodic oxidation, photocatalysis, electro-Fenton and photoelectro-Fenton processes [J].
Brillas, E ;
Mur, E ;
Sauleda, R ;
Sanchez, L ;
Peral, J ;
Domenech, X ;
Casado, J .
APPLIED CATALYSIS B-ENVIRONMENTAL, 1998, 16 (01) :31-42
[8]   Analysis of sonolytic degradation products of azo dye Orange G using liquid chromatography-diode array detection-mass spectrometry [J].
Cai, Meiqiang ;
Jin, Micong ;
Weavers, Linda K. .
ULTRASONICS SONOCHEMISTRY, 2011, 18 (05) :1068-1076
[9]  
Chacko J.T., 2011, J ENVIRON SCI, V1, P1250
[10]   Study on the photocatalytic degradation of methyl orange in water using Ag/ZnO as catalyst by liquid chromatography electrospray ionization ion-trap mass spectrometry [J].
Chen, Tianwen ;
Zheng, Yuanhui ;
Lin, Jin-Ming ;
Chen, Guonan .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2008, 19 (07) :997-1003