Efficient conditional mutation of the vertebrate CENP-C gene

被引：90

作者：

Fukagawa, T

Brown, WRA

机构：

[1] Cancer Res. Campaign Chromosome M., Biochemistry Department, Oxford University, Oxford OX1 3QU, South Parks Road

来源：

HUMAN MOLECULAR GENETICS | 1997年 / 6卷 / 13期

基金：

日本学术振兴会;

关键词：

D O I：

10.1093/hmg/6.13.2301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used gene targeting in the DT40 cell line to create a cell line which expresses a fusion between CENP-C and a mouse steroid receptor and which behaves as a conditional loss of function mutant of CENP-C. Under restrictive conditions these cells arrest at the metaphase/anaphase junction and after a delay of similar to 2.5 h die by apoptosis. These results indicate that CENP-C is either necessary for anaphase chromosome movement or for mediating a signal which triggers centromere function during anaphase. Our approach is simple and applicable to a wide range of proteins with general cell autonomous functions in vertebrates.

引用

页码：2301 / 2308

页数：8

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