Signal-amplified platform for electrochemical immunosensor based on TiO2 nanotube arrays using a HRP tagged antibody-Au nanoparticles as probe

被引：49

作者：

Gao, Zhi-Da ^{[1
]}

Guan, Fang-Fang ^{[1
]}

Li, Cheng-Yong ^{[2
]}

Liu, Hai-Feng ^{[1
]}

Song, Yan-Yan ^{[1
]}

机构：

[1] Northeastern Univ, Coll Sci, Shenyang 110004, Peoples R China

[2] Nanjing Univ, Coll Chem & Chem Engn, Nanjing 210093, Jiangsu, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 41卷

基金：

中国国家自然科学基金;

关键词：

TiO2; nanotubes; Au nanoparticles; Immunosensor; Signal amplification; Platform; TUMOR-NECROSIS-FACTOR; IMMUNOASSAY; DNA; ELECTRODE; ANATASE; LABELS; SERUM; TUBES; WATER;

D O I：

10.1016/j.bios.2012.10.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study, a novel signal-amplified electrochemical immunosensor was proposed by using TiO2 nanotube (TiNT) arrays as the platform. Due to the distinct tubular features-large surface area, high pore volume and good electrochemical conductivity, the TiNT based electrodes exhibited excellent signal-amplified effects. gold nanoparticle (AuNP) was further utilized to bind horseradish peroxidase (HRP) tagged antibodies as recognition elements. Compared to the immunosensor based on either flat electrode, the immunosensors using TiNT layer as electrode showed higher amplified electrochemical signals from the catalytic reaction of HRP relative to hydrogen peroxide (H2O2). Under optimal conditions, the proposed immunosensor exhibited a good electrochemical behavior to antigen in a concentration range from 0.1 ng mL(-1) to 10(5) ng mL(-1) with a detection limit of 0.01 ng mL(-1). The results showed that the TiNT-based electrochemical immunosensing platform could provide a great potential in clinical application for detection of low-abundant proteins. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：771 / 775

页数：5

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