NF-κB activation by equine arteritis virus is MyD88 dependent and promotes viral replication

被引：7

作者：

Mottahedin, Amin ^{[1
,2
]}

Paidikondala, Maruthibabu ^{[3
]}

Cholleti, Harindranath ^{[1
]}

Baule, Claudia ^{[1
]}

机构：

[1] Natl Vet Inst, Dept Virol Immunobiol & Parasitol, SE-75189 Uppsala, Sweden

[2] Uppsala Univ, Infect Biol Grad Program, Dept Med Biochem & Microbiol, S-75123 Uppsala, Sweden

[3] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland

来源：

ARCHIVES OF VIROLOGY | 2013年 / 158卷 / 03期

基金：

瑞典研究理事会;

关键词：

RELA; ARTERIVIRUS; EXPRESSION; INFECTION; SUBUNIT; PATHWAY; IKK;

D O I：

10.1007/s00705-012-1515-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

NF-kappa B, a family of transcription factors involved in different cell functions and immune responses is targeted by viruses. The mechanism of NF-kappa B signalling and its role in replication of EAV have not been investigated. We demonstrate that EAV infection in BHK-21 cells activates NF-kappa B, and this activation was found to be mediated through the MyD88 pathway. Infection of IKK beta(-/-) murine embryo fibroblasts (MEFs), which are deficient in NF-kappa B signalling, resulted in lower virus titre, less cytopathic effect, and reduced expression of viral proteins. These findings implicate the MyD88 pathway in EAV-induced NF-kappa B activation and suggest that NF-kappa B activation is essential for efficient replication of EAV.

引用

收藏

页码：701 / 705

页数：5

相关论文

共 22 条

[1] Toll-like receptor signalling [J].

Akira, S ;

Takeda, K .

NATURE REVIEWS IMMUNOLOGY, 2004, 4 (07) :499-511

[2] RelA Ser276 Phosphorylation-Coupled Lys310 Acetylation Controls Transcriptional Elongation of Inflammatory Cytokines in Respiratory Syncytial Virus Infection [J].

Brasier, Allan R. ;

Tian, B. ;

Jamaluddin, M. ;

Kalita, Mridul K. ;

Garofalo, Roberto P. ;

Lu, Muping .

JOURNAL OF VIROLOGY, 2011, 85 (22) :11752-11769

[3]

Cavanagh D, 1997, ARCH VIROL, V142, P629

[4] Murine Gamma-Herpesvirus 68 Hijacks MAVS and IKKβ to Initiate Lytic Replication [J].

Dong, Xiaonan ;

Feng, Hao ;

Sun, Qinmiao ;

Li, Haiyan ;

Wu, Ting-Ting ;

Sun, Ren ;

Tibbetts, Scott A. ;

Chen, Zhijian J. ;

Feng, Pinghui .

PLOS PATHOGENS, 2010, 6 (07) :1-16

[5] Myeloid differentiation factor-88 plays a crucial role in the pathogenesis of coxsackievirus B3-induced myocarditis and influences type I interferon production [J].

Fuse, K ;

Chan, G ;

Liu, YA ;

Gudgeon, P ;

Husain, M ;

Chen, MY ;

Yeh, WC ;

Akira, S ;

Liu, PP .

CIRCULATION, 2005, 112 (15) :2276-2285

[6] Equine arteritis virus: A review of clinical features and management aspects [J].

Glaser, AL ;

deVries, AAF ;

Rottier, PJM ;

Horzinek, MC ;

Colenbrander, B .

VETERINARY QUARTERLY, 1996, 18 (03) :95-99

[7] Hostile takeovers:: viral appropriation of the NF-κB pathway [J].

Hiscott, J ;

Kwon, H ;

Génin, P .

JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (02) :143-151

[8] Replication of Theiler's virus requires NF-κB-activation:: Higher viral replication and spreading in astrocytes from susceptible mice [J].

Kang, Min Hyung ;

So, Eui Young ;

Park, Hyesuk ;

Kim, Byung S. .

GLIA, 2008, 56 (09) :942-953

[9] Porcine arterivirus activates the NF-κB pathway through IκB degradation [J].

Lee, SM ;

Kleiboeker, SB .

VIROLOGY, 2005, 342 (01) :47-59

[10] The IKKβ subunit of IκB kinase (IKK) is essential for nuclear factor κB activation and prevention of apoptosis [J].

Li, ZW ;

Chu, WM ;

Hu, YL ;

Delhase, M ;

Deerinck, T ;

Ellisman, M ;

Johnson, R ;

Karin, M .

JOURNAL OF EXPERIMENTAL MEDICINE, 1999, 189 (11) :1839-1845