Antioxidant Activity of Extracts from Apple, Chokeberry and Strawberry

被引:13
作者
Bonarska-Kujawa, Dorota
Sarapuk, Janusz
Bielecki, Krzysztof
Oszmianski, Jan
Kleszczynska, Halina
机构
[1] Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, ul. Norwida 25
[2] Department of Fruit Vegetable and Grain Technology, Wrocław University of Environmental and Life Sciences, ul. Norwida 25
[3] Department of Plant Nutrition, Wrocław University of Environmental and Life Sciences, ul. Norwida 25
关键词
HUMAN ERYTHROCYTES; IN-VITRO; PHENOLIC-COMPOUNDS; HUMAN PLASMA; CAPACITY; FRUITS; FLAVONOIDS; ANTHOCYANIN; LEAVES; EFFICIENCY;
D O I
10.2478/v10222-012-0055-7
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The aim of the study was to determine the protective action of extracts from apple, strawberry and chokeberry with respect to linoleic acid and the biological membrane exposed to oxidation induced by physicochemical factors. The activity of the extracts was determined by measuring inhibition of lipid oxidation in red blood cell membrane, induced with UVC radiation and the AAPH radical. The protective effect of the extracts was essayed fluorimetrically and spectrophotometrically. These results together with the ones obtained earlier explain the mechanism of the interaction between the extracts and the red blood cell membrane. The mechanism consists in the incorporation into the membrane and screening the cell against oxidation. The results indicate that the extracts possess very good antioxidant properties, since at the highest concentrations used (0.1 mg/mL) they protect the biological membranes almost entirely against oxidation. Among the extracts studied the best antioxidant properties were exhibited by the apple fruit, which gave 80% or 100% protection of the membrane at 0.05 mg/mL concentration of dry matter, for UVC and AAPH inductors, respectively.
引用
收藏
页码:229 / 234
页数:6
相关论文
共 37 条
[1]   Anthocyanin from strawberry (Fragaria ananassa) with the novel aglycone, 5-carboxypyranopelargonidin [J].
Andersen, OM ;
Fossen, T ;
Torskangerpoll, K ;
Fossen, A ;
Hauge, U .
PHYTOCHEMISTRY, 2004, 65 (04) :405-410
[2]   Human erythrocytes as a system for evaluating the antioxidant capacity of vegetable extracts [J].
Arbos, Kettelin A. ;
Claro, Ligia M. ;
Borges, Lucielly ;
Santos, Cid A. M. ;
Weffort-Santos, Almeriane M. .
NUTRITION RESEARCH, 2008, 28 (07) :457-463
[3]   Development and validation of fluorescence spectroscopic assays to evaluate antioxidant efficacy. Application to metal chelators [J].
Arora, A ;
Strasburg, GM .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1997, 74 (09) :1031-1040
[4]   Formation of flavonoids, especially anthocyanin and chlorogenic acid in 'Jonagold' apple skin: influences of growth regulators and fruit maturity [J].
Awad, MA ;
de Jager, A .
SCIENTIA HORTICULTURAE, 2002, 93 (3-4) :257-266
[5]   Concentration dependent antioxidant/pro-oxidant activity of curcumin Studies from AAPH induced hemolysis of RBCs [J].
Banerjee, Arnab ;
Kunwar, A. ;
Mishra, B. ;
Priyadarsini, K. I. .
CHEMICO-BIOLOGICAL INTERACTIONS, 2008, 174 (02) :134-139
[6]  
Bartosz G., 2003, DRUGA TWARZ TLENU, P15
[7]  
Bonarska-Kujawa D, 2011, J MED PLANTS RES, V5, P2339
[8]   Changes Caused by Fruit Extracts in the Lipid Phase of Biological and Model Membranes [J].
Bonarska-Kujawa, Dorota ;
Pruchnik, Hanna ;
Oszmianski, Jan ;
Sarapuk, Janusz ;
Kleszczynska, Halina .
FOOD BIOPHYSICS, 2011, 6 (01) :58-67
[9]   Fenton reaction applied for screening natural antioxidants [J].
Caillet, Stephane ;
Yu, Hanling ;
Lessard, Stephan ;
Lamoureux, Gilles ;
Ajdukovic, Djordje ;
Lacroix, Monique .
FOOD CHEMISTRY, 2007, 100 (02) :542-552
[10]   Interaction of flavonoids with red blood cell membrane lipids and proteins: Antioxidant and antihemolytic effects [J].
Chaudhuri, Sudip ;
Banerjee, Anwesha ;
Basu, Kaushik ;
Sengupta, Bidisa ;
Sengupta, Pradeep K. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2007, 41 (01) :42-48