Comprehensive Analysis of Silencing Mutants Reveals Complex Regulation of the Arabidopsis Methylome

被引：623
作者：
Stroud, Hume [1 ]
Greenberg, Maxim V. C. [1 ]
Feng, Suhua [1 ,2 ,3 ]
Bernatavichute, Yana V. [1 ]
Jacobsen, Steven E. [1 ,2 ,3 ]
机构：
[1] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA
来源：
CELL | 2013年 / 152卷 / 1-2期
基金：
美国国家科学基金会;
关键词：
NOVO DNA METHYLATION; LOCUS-SPECIFIC CONTROL; RNA-POLYMERASES IV; TRANSPOSABLE ELEMENTS; THALIANA; GENES; CHROMATIN; GENOME; METHYLTRANSFERASE; HETEROCHROMATIN;
D O I：
10.1016/j.cell.2012.10.054
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cytosine methylation is involved in various biological processes such as silencing of transposable elements (TEs) and imprinting. Multiple pathways regulate DNA methylation in different sequence contexts, but the factors that regulate DNA methylation at a given site in the genome largely remain unknown. Here we have surveyed the methylomes of a comprehensive list of 86 Arabidopsis gene silencing mutants by generating single-nucleotide resolution maps of DNA methylation. We find that DNA methylation is site specifically regulated by different factors. Furthermore, we have identified additional regulators of DNA methylation. These data and analyses will serve as a comprehensive community resource for further understanding the control of DNA methylation patterning.
引用
收藏
页码：352 / 364
页数：13
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