Variation and intraspecific relationships in Indian wild Musa balbisiana (BB) population as evidenced by random amplified polymorphic DNA

被引:32
|
作者
Uma, S [1 ]
Siva, SA [1 ]
Saraswathi, MS [1 ]
Manickavasagam, M [1 ]
Durai, P [1 ]
Selvarajan, R [1 ]
Sathiamoorthy, S [1 ]
机构
[1] Natl Res Ctr Banana, Div Crop Improvement, Tiruchirappalli 620102, Tamil Nadu, India
关键词
intraspecific relationship; molecular characterization; morphotaxonomic characterization; Musa balbisiana; polymorphism; RAPD;
D O I
10.1007/s10722-004-0576-y
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Sixteen collections of the wild Musa species, Musa balbisiana Colla collected from different regions of India were studied for their intraspecific relationships using random amplified polymorphic DNA (RAPD) markers. Out of 80 primers screened, 34 primers produced reproducible bands and four primers among them showing polymorphic bands were used. In all, 43 DNA fragments were amplified averaging 10.75 per primer. Of these, 31 amplified fragments showed polymorphism (averaging of 7.75 per primer). The extent of polymorphism (74.6%) has indicated the existence of considerable variation at the DNA level within the species. The 16 accessions were clustered into four as against seven clusters obtained through morphotaxonomic characterization. The inter relationships based on geographical origin in comparison with molecular characterization have been discussed.
引用
收藏
页码:349 / 355
页数:7
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