Apelin-APJ induces ICAM-1, VCAM-1 and MCP-1 expression via NF-κB/JNK signal pathway in human umbilical vein endothelial cells

被引：69

作者：

Lu, Ying ^{[2
]}

Zhu, Xiao ^{[1
]}

Liang, Gan-Xiong ^{[2
]}

Cui, Rong-Rong ^{[1
]}

Liu, Yuan ^{[1
]}

Wu, Shan-Shan ^{[1
]}

Liang, Qiu-Hua ^{[1
]}

Liu, Guan-Ying ^{[1
]}

Jiang, Yi ^{[1
,3
]}

Liao, Xiao-Bo ^{[4
]}

Xie, Hui ^{[1
]}

Zhou, Hou-De ^{[1
]}

Wu, Xian-Ping ^{[1
]}

Yuan, Ling-Qing ^{[1
]}

Liao, Er-Yuan ^{[1
]}

机构：

[1] Cent S Univ, Xiang Ya Hosp 2, Inst Metab & Endocrinol, Changsha 410011, Hunan, Peoples R China

[2] Peoples Hosp Zhongshan City, Dept Endocrinol, Zhongshan 528403, Guangdong, Peoples R China

[3] Cent S Univ, Xiangya Hosp 2, Dept Pathol, Changsha 410011, Hunan, Peoples R China

[4] Cent S Univ, Xiangya Hosp 2, Dept Cardiothorac Surg, Changsha 410011, Hunan, Peoples R China

来源：

AMINO ACIDS | 2012年 / 43卷 / 05期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Apelin; Human umbilical vein endothelial cells; Intercellular adhesion molecule-1; Vascular cell adhesion molecule-1; Monocyte chemotactic protein-1; MONOCYTE CHEMOATTRACTANT PROTEIN-1; INTERCELLULAR-ADHESION MOLECULE-1; VASCULAR INFLAMMATION; HEART-FAILURE; C-JUN; X-MSR; RECEPTOR; ATHEROSCLEROSIS; ANGIOGENESIS; MECHANISMS;

D O I：

10.1007/s00726-012-1298-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apelin receptor (APJ) deficiency has been reported to be preventive against atherosclerosis. However, the mechanism of this effect remains unknown. In this study, quantitative real-time RT-PCR, Western blotting and ELISA analyses revealed a significant increase in the expression of intercellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1) in human umbilical vein endothelial cells (HUVECs) treated with apelin. Inhibitors of cellular signal transduction molecules were used to demonstrate involvement of nuclear factor kappa-B (NF-kappa B) and c-Jun N-terminal kinase (JNK) pathways in apelin-APJ-induced activation of adhesion molecules and chemokines. Inhibition of APJ expression by RNA interference abrogated apelin-induced expression of adhesion molecules and chemokines and apelin-stimulated cellular signal transduction in HUVECs. The apelin-APJ system in endothelial cells is involved in the expression of adhesion molecules and chemokines, which are important for the initiation of endothelial inflammation-related atherosclerosis. Therefore, apelin-APJ and the cell signaling pathways activated by this system in endothelial cells may represent targets for therapy of atherosclerosis.

引用

页码：2125 / 2136

页数：12

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