Sports practice spirit in Yanan period is an important part of Yanan spirit, it is very meaningful for us to develop sports and build harmonious society by inheriting and carrying forward the spirits in new period. First of all, the paper explained the connotation and denotation of Yanan sports practice spirit. The connotation was the experience and knowledge that the CPC concluded in developing sports industry in Yanan period, and the denotation was the outward manifestation of the connotation, which included a method to pursuit ideal, serving the people heart and soul, relying on one's own efforts, working hard and perseveringly, seeking truth from facts, and collectivism and so on. Based on understanding deeply its connotation and denotation, and combined with the reality situations of China, the research analyzed seriously its contemporary values. The conclusions show that the contemporary value is reflected in three aspects. Firstly, it can provide experiences for making sports policies in new era. Secondly, it can help to promote Chinese sports spirit in contemporary society, and finally it can provide demonstration for carrying out the national fitness in harmonious society.