A high contrast method of unstained biological samples under a thin carbon film by scanning electron microscopy

被引：15

作者：

Ogura, Toshihiko ^{[1
,2
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Neurosci Res Inst, Tsukuba, Ibaraki 3058568, Japan

[2] Japan Sci & Technol Agcy, PRESTO, Saitama, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 377卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

Scanning election microscopy; Biological sample; Bacteria; Secondary election; Low acceleration voltage; Carbon film; Lower surface;

D O I：

10.1016/j.bbrc.2008.09.097

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The contrast of biological samples in scanning electron microscopy (SEM) is very weak. To examine a biological specimen by SEM, many steps and/or special equipment are required to prepare the sample. Here, we describe a method using an Unstained biological sample Under a 40 nm carbon film to give a high contrast image, where the image is detected by the secondary electron (SE) signal at a low accelerating volt,e of 1.5 kV. Under these conditions, it is hard to detect a direct signal front a biological specimen. The age high contrast image is created by the SEs from the lower surface of the thin carbon film. Therefore, the damage to the sample from the electron beam is very low. Our method can be utilized to observe various biological samples of bacteria, viruses. and protein complexes. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：79 / 84

页数：6

共 24 条

[1] Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation [J].