AKT activator SC79 protects hepatocytes from TNF-α-mediated apoptosis and alleviates D-Gal/LPS-induced liver injury

被引:84
作者
Jing, Zhen-Tang [1 ]
Liu, Wei [1 ,2 ]
Xue, Chao-Rong [1 ]
Wu, Shu-Xiang [1 ]
Chen, Wan-Nan [1 ,2 ]
Lin, Xin-Jian [1 ]
Lin, Xu [1 ,2 ]
机构
[1] Fujian Med Univ, Sch Basic Med Sci, Key Lab, Minist Educ Gastrointestinal Canc, Fuzhou, Fujian, Peoples R China
[2] Fujian Med Univ, Fujian Key Lab Tumor Microbiol, Dept Med Microbiol, Fuzhou, Fujian, Peoples R China
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2019年 / 316卷 / 03期
基金
中国国家自然科学基金;
关键词
AKT activation; Akt activator SC79; hepatocyte apoptosis; liver injury; TNF-alpha; TNFR1; TUMOR-NECROSIS-FACTOR; KAPPA-B ACTIVATION; CELL-DEATH; PHOSPHATIDYLINOSITOL; 3-KINASE/AKT; HEPATOCELLULAR APOPTOSIS; MOUSE HEPATOCYTES; FACTOR RECEPTORS; C-FLIP; FAS; SIGNALS;
D O I
10.1152/ajpgi.00350.2018
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Tumor necrosis factor-alpha (TNF-alpha) is a highly pleiotropic cytokine executing biological functions as diverse as cell proliferation, metabolic activation, inflammatory responses, and cell death. TNF-alpha can induce multiple mechanisms to initiate apoptosis in hepatocytes leading to the subsequent liver injury. Since the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathway is known to have a protective role in death factor-mediated apoptosis, it is our hypothesis that activation of Akt may represent a therapeutic strategy to alleviate TNF-alpha-induced hepatocyte apoptosis and liver injury. We report here that the Akt activator SC79 protects hepatocytes from TNF-alpha-induced apoptosis and protects mice from D-galactosamine (D-Gal)/lipopolysaccharide (LPS)-induced TNF-alpha-mediated liver injury and damage. SC79 not only enhances the nuclear factor-kappa B (NF-kappa B) prosurvival signaling in response to TNF-alpha stimulation, but also increases the expression of cellular FLICE (FADD-like IL-1 beta-converting enzyme)-inhibitory protein L and S (FLIPL/S), which consequently inhibits the activation of procaspase-8. Furthermore, pretreatment of the PI3K/Akt inhibitor LY294002 reverses all the SC79-induced hepatoprotective effects. These results strongly indicate that SC79 protects against TNF-alpha-induced hepatocyte apoptosis and suggests that SC79 is likely a promising therapeutic agent for ameliorating the development of liver injury. NEW & NOTEWORTHY SC79 protects hepatocytes from TNF-alpha-mediated apoptosis and mice from Gal/LPS-induced liver injury and damage. Cytoprotective effects of SC79 against TNF-alpha act through both AKT-mediated activation of NF-kappa B and upregulation of FLIPL/S.
引用
收藏
页码:G387 / G396
页数:10
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