A real-time PCR for the detection of infectious myonecrosis virus in penaeid shrimp

被引：6
作者：
Liu, Hong-Ling [1 ]
Yan, Dong-Chun [1 ,2 ]
Sun, Hu-Shan [3 ]
Wang, Yi-Yan [3 ]
Wang, Lei [3 ]
机构：
[1] Ludong Univ, Coll Agr, Yantai 264025, Peoples R China
[2] Chinese Acad Fisheiy Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
[3] Ludong Univ, Coll Life Sci, Yantai 264025, Peoples R China
来源：
JOURNAL OF INVERTEBRATE PATHOLOGY | 2013年 / 113卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Infectious myonecrosis virus; Real-time PCR; Penaeid shrimp; HISTORIC EMERGENCE; MONODON; IMNV; PATHOGENS; OUTBREAKS; VANNAMEI; IMPACT; ASSAY;
D O I：
10.1016/j.jip.2013.04.009
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
Infectious myonecrosis virus (IMNV) is a recently observed shrimp virus, which threats the cultured Litopenaeus vannamei and can cause huge economic loss in shrimp farming industry. The specific aim of this study was to develop a new sensitive real-time PCR method for the specific detection of shrimp IMNV. A real-time PCR assay with a pair of primers to specifically amplify a 101 bp IMNV cDNA fragment and a corresponding TaqMan probe was developed, which shown to be specific for IMNV without cross reaction with DNA samples prepared from four other shrimp viruses including white spot syndrome virus (WSSV), hepatopancreatic parvovirus (HPV), monodon baculovirus (MBV), and infectious hypodermal and haematopoietic virus (IHHNV). The method could detect as low as one single copy of IMNV plasmid cDNA. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码：237 / 239
页数：3
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