Production of Xylooligosaccharides from Forest Waste by Membrane Separation and Paenibacillus Xylanase Hydrolysis

被引：0

作者：

Ko, Chun-Han ^{[1
,2
]}

Shih, Tzenge-Lien ^{[3
]}

Jhan, Bi-Ting ^{[1
]}

Chang, Fang-Chih ^{[4
]}

Wang, Ya-Nang ^{[1
]}

Wang, Yi-Chung ^{[5
]}

机构：

[1] Natl Taiwan Univ, Sch Forestry & Resource Conservat, Taipei 10617, Taiwan

[2] Natl Taiwan Univ, Bioenergy Res Ctr, Taipei 10617, Taiwan

[3] Tamkang Univ, Dept Chem, Tamsui 25137, Taipei County, Taiwan

[4] Natl Cheng Kung Univ, Dept Environm Engn, Tainan 70101, Taiwan

[5] Chinese Culture Univ, Dept Forestry & Nat Conservat, Taipei 11114, Taiwan

来源：

BIORESOURCES | 2013年 / 8卷 / 01期

关键词：

Mikania micrantha; Xylooligosaccharides; Paenibacillus; Xylanase; Membrane separation; Nanofiltration; XYLO-OLIGOSACCHARIDES; ENZYMATIC PRODUCTION; CAMPINASENSIS BL11; CORNCOB XYLAN; AUTOHYDROLYSIS; PURIFICATION;

D O I：

暂无

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

Xylooligosaccharides (XO), derived from the alkaline (NaOH) extractant of Mikania micrantha, were produced using multiple staged membrane separation and enzymatic xylanolysis. Staged nanofiltration (NMX), ultrafiltration (EUMX), and centrifugation (EMX) processes for the ethanol precipitates were conducted. NMX recovered 97.26% of total xylose and removed 73.18% of sodium ions. Concentrations of total xylose were raised from 10.98 to 51.85 mg/mL by the NMX process. Recovered xylan-containing solids were hydrolyzed by the recombinant Paenibacillus xylanase. 68% XO conversions from total xylose of NMX was achieved in 24 hours. Xylopentaose (DP 5) was the major product from NMX and EMX hydrolysis. Xylohexaose (DP 6) was the major product from EUMX hydrolysis. Results of the present study suggest the applicability for XO production by nanofiltration, as NMX gave higher XO yields compared to those from a conventional ethanol-related lignocellulosic waste conversion process.

引用

页码：612 / 627

页数：16

共 33 条

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